茶籽油DNA提取方法的比较

Comparison of different methods for extracting DNA from camellia oil

针对食用油脂中DNA含量极低、DNA序列片段短、破坏严重的特点,通过实时荧光PCR法检测植物的通用tRNALeu,对茶籽油DNA几种提取方法进行比较,建立了食用油脂中DNA提取新方法。结果证明:较之于文献中常见植物油DNA几种提取方法,用改进方法提取的DNA含量高、纯度高,可以作为PCR反应的模板,为食用油脂进行核酸类生物性检测提供了一种简捷有效的方法。

A new method for extracting DNA from edible oil was established by comparing several methods, since DNA in edible oil had the feature of low content, short fragment and being seriously destroyed. The tRNALeu could be detected in the edible oil by the real - time PCR method. Results showed that the improved method could extract DNA in higher content and purity compared with common methods suggested in reports, which can be used as the PCR template to provide a simple and effective method for detecting nucleic acids substance in edible oil.