GBEE对荷瘤小鼠IL-2、IL-12、TNF-α及TGF-α水平的影响

The effect of GBEE on interleukin-2,interleukin-12,tumor necrosis factor-α and transforming growth factor-α level in S180 tumor-bearing mice

目的:研究银杏外种皮提取物(GBEE)的抗肿瘤作用及其对荷瘤小鼠免疫功能的影响。方法:采用ICR小鼠,建立S180移植瘤模型,GBEE连续灌胃16天,观察荷瘤小鼠移植瘤抑制率;体外培养荷瘤小鼠脾脏细胞,应用LPS诱生IL-12,应用ConA诱生IL-2;采用双抗体夹心ABC-ELISA法测定IL-12及荷瘤小鼠血清TNF-α含量;采用MTT法检测IL-2活性;采用放射免疫分析法测定荷瘤小鼠血清TGF-α的含量。结果:GBEE可明显抑制小鼠S180移植瘤的生长,其中200 mg/(kg.d)剂量组抑瘤率达38.36%;GBEE可提高荷瘤小鼠血清TNF-α含量,促进脾淋巴细胞IL-12的形成,增强脾脏T淋巴细胞IL-2活性,降低荷瘤小鼠血清TGF-α水平。结论:GBEE可抑制瘤细胞分泌TGF-α,具有显著抗肿瘤作用;可促进细胞因子IL-2、IL-12和TNF-α的生成,具有增强荷瘤机体免疫功能的作用。GBEE的免疫增强效应可能是其发挥抗肿瘤作用的重要途径之一。

Objective：To study the anti-tumor function and immune effect in tumor-bearing mice of Ginkgo biloba exocarp extracts（GBEE）.Methods：S180 sarcoma transplantable tumor model of ICR mice was established.GBEE was given ig（intragastrical administration）16 days,the inhibitory rate of transplantable tumor in mice was observed;spleen cells of the tumor-bearing mice were cultared in vitro,IL-2 was induced by ConA and IL-12 was induced by LPS.The level of IL-12 and TNF-α,the activity of IL-2 and the serum content of transforming growth factor-α（TGF-α） were evaluated by enzyme-linked immunosorbent assay,MTT assay and radioimmunoassay,respectively.Results：GBEE significantly inhibited S180 transplantable tumor growth in vivo,with the inhibitory rate of 200 mg/（kg·d） was 38.36%;GBEE significantly increased the production of TNF-α and IL-12,decreased the content of TGF-α and promoted the activity of IL-2 in tumor-bearing mice.Conclusion：GBEE inhibits the secretion of TGF-α by tumor cells,has significant anti-tumor effect;GBEE increases the production of IL-2,IL-12 and TNF-α,enhances the immune function of tumor-bearing organism.The anti-tumor effect of GBEE may be mediated by inducing the anti-tumor immunity of the host.