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三株H9N2亚型禽流感病毒全基因组序列分析 被引量：5

Sequence Analysis of Three H9N2 Avian Influenza Virus Strains Isolated from South China
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摘要 采用RT-PCR对3株H9N2亚型禽流感病毒基因组进行扩增,测序后再利用DNAStar和MEGA 4对所得到的序列和部分具有代表性的参考序列进行遗传演化及分子特点分析,旨在从分子水平探讨华南地区H9N2亚型禽流感病毒的流行特点,为该病的综合防控提供依据。结果表明,3个分离株CK/GD/162/03、Francolin/GD/298/05与CK/GD/A8/10分别属于B34、B47和B54基因型;3个分离株HA基因未发生明显的变异,符合我国大陆H9N2亚型AIV HA基因的遗传进化特点,但内部基因出现不同程度重组且部分基因与人源高致病性H5N1亚型AIV同源性很高;CK/GD/162/03、Francolin/GD/298/05两株分离株具有宿主多样性。因此,对华南地区H9N2亚型AIV进行监控及分子流行病学调查具有重要意义。 Full length cDNAs of the whole genome of three avian influenza virus strains were amplified by RT-PCR and were sequenced.Then,DNA Star and MEGA4 were used to study the phylogenetic and molecular characterization of H9N2 AIV in south China in order to provide the deta support for this virus prevention.The results showed that CK/GD/162/03,Francolin/GD/298/05 and CK/GD/A8/10 belonged to B34,B47and B54 subtype.It was shown that these viruses exhibited the common feature of H9 subtype AIV from China,but the internal genes were reassorted,and two viruses（CK/GD/162/03,Francolin/GD/298/05） owed many hosts.Therefore,the continuing evolution of H9N2 influenza viruses emphasizes the importance of H9N2 influenza virus surveillance and molecular epidemiology investigation in south China.
作者 刘国乾 曹蓝 和君 冯军祥 张长辉 田进 焦培荣 亓文宝 廖明
机构地区 华南农业大学兽医学院
出处 《动物医学进展》 CSCD 北大核心 2012年第5期1-7,共7页 Progress In Veterinary Medicine
基金 国家肉鸡产业技术体系项目(nycytx-42-G3-03) 广东省自然科学基金项目(10251064201000004和10151064201000021) 国家自然基金项目(31172343) 广东省科技计划项目(20100206和2010B020307005)
关键词 禽流感病毒 H9N2亚型 基因重组 遗传演化 Aivan influenza virus H9N2 subtype gene reassortment phylogenetic characterization
分类号 S852.659.5 [农业科学—基础兽医学]
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参考文献29

  • 1Guo Y J, Krauss S, Senne D A, et al. Characterization of the pathogenicity of members of the newly established HgN2 influenza virus lineages in Asia[J]. Virology, 2000,267 (2) : 279- 288.
  • 2Sun Y, Pu J, Jiang Z, et al. Genotypic evolution and antigenic drift of H9N2 influenza viruses in China from 1994 to 2008 [J]. Vet Microbiol,2010,146(3-4):215-225.
  • 3Xu K M, Smith G J, Bahl J, et al. The genesis and evolution of H9N2 influenza viruses in poultry from southern China, 2000 to2005[J]. J Virol,2007,81(19):10389-10401.
  • 4Xu X J, Xu G Y, Zhou H B, et al. Evolutionary characterization of influenza virus A/duck/Hubei/Wl/2004 (H9N2) isolated from central China[J]. Virus Genes, 2008,36 (1) : 79-83.
  • 5Liu J H, Okazaki K, Shi W M, et al. Phylogenetic analysis of hemagglutinin and neuraminidase genes of HgN2 viruses isolated from migratory ducks[J]. Virus Genes, 2003,27 (3) : 291- 296.
  • 6Yu H, Zhou Y J, Li G X, et al. Genetic diversity of HgN2 influenza viruses from pigs in China: a potential threat to human health? [J]. Vet Microbiol,2011,149(1-2) :254-261.
  • 7Lin Y P, Shaw M, Gregory V, et al. Avian-to-human transmission of H9N2 subtype influenza A viruses: relationship between HgN2 and H5N1 human isolates[J]. Proc Natl Acad Sci U S A,2000,97(17) :9654-9658.
  • 8Peiris M, Yuen K Y, Leung C W, et al. Human infection with influenza H9N2[J]. Lancet,1999,354(9182) :916-917.
  • 9Guan Y, Shortridge K F, Krauss S, et al. H9N2 influenza vi ruses possessing HSN1-like internal genomes continue to circulate in poultry in southeastern China[J]. J Virol, 2000, 74 (20) :9372-9380.
  • 10齐岩,袁润余,张贺楠,亓文宝,单芬,胡玥,李小康,焦培荣,廖明.H9N2亚型禽流感病毒广东分离株的全基因克隆及序列分析[J].病毒学报,2010,26(3):176-182. 被引量：6

二级参考文献28

  • 1陈伯伦,张泽纪,陈伟斌.禽流感研究I．鸡A型禽流感病毒的分离与血清学初步鉴定[J].中国兽医杂志,1994,20(10):3-5. 被引量：237
  • 2Webster R G, Bean W J, Gorman O T, et al. Evolution and ecology of influenza A viruses[J]. Microbiol Rev, 1992, 56 (1): 152-179.
  • 3Fouchier R A M, Munster V, Wallensten A, et al Characterization of a novel influenza A virus hemaggluti nin subtype (H16) obtained from black-headed gulls[J] J Virol, 2005, 79(5): 2814-2822.
  • 4Subbaral K, Joseph T. Scientific barriers to developing vaccines against avian influenza viruses[J]. Nat Rev Immunol, 2007, 7(4): 267-278.
  • 5Guan Y, Shortridge K F, Krauss S, et al. Molecular characterization of H9N2 influenza viruses: were they the donors of the "internal" genes of H5N1 viruses in Hong Kong[J]. Proc Natl Acad Sci USA, 1999, 96(16): 9363-9367.
  • 6Kumar S, Tamura K, Nei M. MEGA 3: an integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment[J]. Brief Bioinform, 2004, 5 (2) : 150-163.
  • 7Nobusawa E, Aoyama T, Kato H, et al. Comparison of complete amino acid sequences and receptor-binding proteins among 13 serotypes of hemagglutininsof influenza A viruses[J]. Virology, 1991, 182(2): 475-485.
  • 8Lin Y P, Shaw M, Gregory V, et al. Avian-to-human transmission of H9N2 subtype influenza A viruses: Relationship between HgN2 and H5N1 human isolates[J]. Proe Natl Acad Sci USA, 2000, 97(17): 9654-9658.
  • 9http://www, cbs. dtu. dk/services/NetNGlyc-1. 0/ [DB].
  • 10Choi Y K, Ozaki H, Webby R J, et al. Continuing evolution of H9N2 influenza viruses in Southeastern China [J]. J Virol, 2004, 78(16):8609-8614.

共引文献5

同被引文献49

  • 1廖明.禽病防控的关键问题探讨[J].中国家禽,2012,34(07):36-38.
  • 2Liu X, Ma H, Xu Q, et al. Characterization of a recombi- nant coronavims infectious bronchitis virus with distinct S1 sub- units of spike and nucleocapsid genes and a 3' untranslated re- gion[J]. Vet Microbiol,2012,162(2-4) :429-436.
  • 3Liu D, Zhang X B, Yan Z Q, et al. Molecular characteriza- tion and phylogenetic analysis of infectious bursal disease virus- es isolated from chicken in South China in 2011[J]. Trop An- im Health Prod,2012,DOI 10.1007/s11250-012-0333-8.
  • 4Gao Y,Yun B, Qin L,et al. Molecular epidemiology of avi- an leukosis virus subgroup J in layer flocks in China[J].J Clin Microbiol, 2012,50(3) : 953-960.
  • 5Aric8 E, Belardelli F. Interferon-or as antiviral and antitu- mor vaccine adjuvants: mechanisms of action and response signa- ture[J].J Interferon Cytokine Res,2012,32(6):235-247.
  • 6Omarov R T, Scholthof H B. Biological chemistry of vi- res-encoded suppressors of RNA silencing: an overview [J]. Methods Mol Biol,2012,894:39-56.
  • 7Gao Rong-bao, Cao Bin, Hu Yun-wen, et al. Human infection with a novel avian-origin Influenza A (H7N9) virus [J]. N Engl J Med, 2013, 368(20): 1888-1897.
  • 8Hoffmann E, Stech J, Guan Y, et al. Universal primer set for the full-length amplification of all influenza A viruses [J]. Arch Virol, 2001, 146(12): 2275-2289.
  • 9Matrosovich M N, Krauss S, Webster R G. H9N2 influenza A viruses from poultry in Asia have human virus-like receptor specificity [J]. Virology, 2001, 281(2): 156-162.
  • 10Sun Y, Tan Y, Wei K, et al. Amino acid 316 of hemagglutinin and the neuraminidase stalk length influence virulence of H9N2 influenza virus in chickens and mice [J]. J Virol, 2013, 87(5): 2963-2968.

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动物医学进展
2012年 第5期

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