小麦磷饥饿前后根系蛋白质组差异表达谱建立及差异表达蛋白的鉴定

Identification of Differential Expressed Proteins Responding to Phosphorus Starvation Based on Proteomic Analysis in Roots of Wheat(Triticum aestivum L.)

以耐低磷的小麦基因型洛夫林10号为材料,采用蛋白质双向电泳技术,结合质谱鉴定,分析了正常磷供应和无磷处理7d后根系中的蛋白质组表达谱差异,以期为深入探讨小麦响应磷胁迫的分子机理提供蛋白水平上的数据和资料。研究发现,在可重复检测到的1144个蛋白点中,有87个在磷胁迫处理前后发生了明显的表达改变,占总数的7.6%,包括磷胁迫前特异表达、磷胁迫后特异表达、磷胁迫后上调和磷胁迫后下调表达等4种差异表达模式。在87个差异表达蛋白点中,有39个通过质谱技术被成功鉴定,涉及到代谢、细胞生长和分裂、转录和翻译、抗病、信号转导、转座元件及未知功能蛋白等功能类别,说明小麦可能通过细胞的代谢状态和基因表达改变来适应磷胁迫,进而维持体内磷含量的平衡状态。最后,还对差异表达点与磷胁迫的关系进行了分析和讨论。

Growth inhibition caused by phosphorus（P）deficiency is a serious problem for crop production.Plants can respond defensively to this stress by modifying their metabolic pathways and root morphologies through changes of quantity of low-Pi responding genes.To better understand the adaptation mechanisms of wheat to Pi deficiency conditions,a comparative proteome analysis was conducted in this study using wheat root samples treated 7 days without（-P）and with phosphorus（＋P）.Among 1 144 protein spots reproducibly detected,eighty seven of which（7.6%）were differentially expressed,including those present in treatments of only in-P or ＋P,and up-or down-regulated in-P.Moreover,39 of the differentially expressed proteins were revealed to be involved in various biological processes such as metabolism,cell growth and division,transcription and translation,disease and defense,signal transduction,acting as transposable elements and unclassified proteins.Taken together,our results showed that wheat responds to the Pi starvation stress through an array of changes in metabolic states of cells and genes expression,which results in the maintenance of a relative P homeostasis in plants.