天精中黄酮类化合物的结构鉴定

Identification on Flavonoids in Leaves of Lycium Chinense

用反相高效液相色谱法，以 Ｓｐｈｅｒｉｓｏｒｂ ＯＤＳ柱为固定相，甲醇一四氢呋喃一水一甲酸为流动相，并应用二极管阵列检测技术，分析了天精中的黄酮类化合物，经ＵＶ、ＩＲ、ＨＮＭＲ、１３ＣＮＭＲ、ＭＳ等波谱分析，鉴定了天精中 ５种黄酮类化合物分别是 ５，７，３’一三羟基一６，４’，５’一三甲氧基黄酮（１），金合欢素（２），金合欢素一７—Ｏ—ａ—Ｌ一鼠李糖基（１→６）一β一Ｄ一葡萄糖甙（３），木樨草素（４），槲皮素一３—Ｏ—ａ—Ｌ一鼠李糖基（１→６）一β—Ｄ一葡萄糖甙（５），其中（１）为新化合物．

Flavonoids in leaves of lycium chinense were analysed by using high performance liquid chromatography in which spherisorb ODS column as stationary phase, methanol - tetrahydrofuran - water - formic acid as mobile phase and diode array detecting technique was used. Five flavoniods were identified as 5, 7, 3 - trihydroxy - 6, 4', 5'- trimethoxy flavone(1 ), acacetin(2 ), acacetin - 7 - O - a - L - rhamnopyranosyl - (l6) - β - D - glucopyranside (3 ), luteolin(4) and quercetin - 3 - O - a - L - rhamnopyranosyl - (1-6) - β - D - glucopyranside(5 ) by means of UV, IR, IHNMR, 13 CNMR and MS.