两种拟南芥MYB51突变体的鉴定与初步分析

Identification and preliminary analysis of two MYB51 Arabidopsis thaliana mutants

拟南芥MYB51(AT1G18570)转录因子是吲哚族芥子油苷合成的正向调控因子。研究组从拟南芥生物资源中心(Arabidopsis Biological Resource Center,Ohio State University,USA)购买AT1G18570的两种T-DNA插入突变体SALK_045103和SALK_059771,分别通过基因型分析筛选得到纯合突变体。半定量RT-PCR分析结果表明,两种突变体中MYB51基因的表达量极低。高效液相色谱检测结果表明,两种突变体中吲哚族芥子油苷含量与野生型相比均显著降低。由此可知,SALK_045103和SALK_059771突变体中MYB51转录因子的功能显著降低,为进一步探讨拟南芥MYB51转录因子在环境因子调控吲哚族芥子油苷合成过程中的作用奠定了材料基础。

Arabidopsis MYB51（AT1G18570） transcription factor is one of the positive regulatory factors of indole glucosinolates synthesis.Two T-DNA insertion mutants of AT1G18570 SALK045103 and SALK059771 were purchased from the Arabidopsis Biological Resource Center（Ohio State University,USA）,and the homozygous mutants were obtained by genotyping analysis.Semi-quantitative RT-PCR analysis showed that the expression of MYB51 gene was significantly reduced in both mutants and HPLC analyses showed that indole glucosinolates were significantly reduced in them.The results indicated that function of MYB51 transcription factor was much inhibited in the SALK045103 and SALK059771 mutants,and both of the mutants could be used for the further study of environmental factors-regulated indole glucosinolates synthesis in Arabidopsis thaliana.