硒和别嘌呤醇对镧损伤小鼠肝的保护作用

Protective effects of selenium and allopurinol on the mouse liver damages induced by lanthanum

小鼠iv LaCl_3 7.5 mg/kg使肝发生明显损伤,ALT显著升高;光镜下见肝细胞变性,PAS染色显示糖原减少或消失;电镜下见胞浆中含许多脂滴,线粒体凝缩,内质网扩张、断裂,粗面内质网上的核糖小体脱落;血清脂质过氧化物水平显著升高。iv LaCl_3前预先给APL或Se保护的动物,ALT的升高程度显著地受到阻抑,光镜和电镜下均见肝细胞的损伤明显减轻,血清脂质过氧化物水平显著降低。结果表明APL和Se对镧引起的肝损伤均有保护作用,可能与它们能降低血清脂质过氧化物的作用有关。

The mice were with iv LaCl3 7.5 mg/kg,and 24 h later the levels of ALT and SLPO(serum lipid peroxide)elevated significantly.Light microscopic observations showed hepatocytes degenerated and PAS stain showed the decrease or disappearance of glycogen in the cytoplasm.Under an electron microscope,many lipid droplets spread in the cytoplasm.Some mitochondria condensed,some endoplasmic reticula dilated or broken,and ribosomes fell off from them.When APL or Se was given beforeLaCl3 the ALT elevation was markedly suppressed,and the damages of hepatocytes were obviously lightened.In most liver sections,PAS stain held normal positive reaction.SLPO level reduced significantly.The results indicate that the protective effects of Se and APL on the liver intoxication induced by LaCl3 in mice may be due to the reduction of SLPO.