曲古抑菌素A对鼻咽癌CNE2细胞凋亡的影响及机制研究

Influence of trichostatin A on cell apoptosis in nasopharyngeal carcinoma CNE2 cells and its mechanism

目的探讨曲古抑菌素A(TSA)对鼻咽癌CNE2细胞凋亡的影响及作用机制。方法以不同浓度(300、400、500 nmol/L)TSA作用鼻咽癌CNE2细胞株,采用流式细胞术测定TSA作用前后鼻咽癌细胞凋亡情况;逆转录聚合酶链反应(RT-PCR)分析TSA作用后鼻咽癌细胞中细胞周期素依赖性蛋白激酶抑制因子1A(P21)表达的变化。结果以300、400、500 nmol/L TSA分别诱导CNE2细胞凋亡,作用24 h后凋亡率分别为12.52%±1.57%、19.69%±3.00%、19.63%±2.68%;48 h后凋亡率为24.50%±4.45%、36.24%±3.92%、34.82%±6.45%;与对照组(0 nmol/L TSA)比较,差异均有统计学意义(P<0.05)。RT-PCR检测发现,TSA可诱导CNE2细胞内p21基因表达明显增加(P<0.01)。结论 TSA能明显诱导CNE2细胞凋亡,其作用机制可能与p21基因的异常表达有关。

Objective To investigate the influence of trichostatin A（TSA） on cell apoptosis in nasopharyngeal carcinoma CNE2 cells and its mechanism.Methods CNE2 cells were incubated with TSA at different concentrations（300,400 and 500 nmol/L）.The effects of TSA on cell apoptosis were detected by flow cytometry.The expression of cyclin-dependent kinase inhibitor 1A（P21） was analyzed by reverse transcription-polymerase chain reaction（RT-PCR）.Results The apoptosis rates with different concentrations of TSA（300,400 and 500 nmol/L） were 12.52%±1.57%,19.69%±3.00% and 19.63%±2.68% after 24 h treatment.The apoptosis rates were 24.50%±4.45%,36.24%±3.92% and 34.82%±6.45% after 48 h treatment.There were statistical significance compared with those of controls（0 nmol/L TSA,P0.05）.The expression level of p21 increased after TSA treatment（P0.01）.Conclusions TSA markedly induces CNE2 cell apoptosis.Its mechanism would be related with the abnormal expression of p21 gene.