甲基丙烯酸环氧丙酯的遗传毒性

Genotoxicity of glycidyl methacrylate

为了评估甲基丙烯酸环氧丙酯(GMA)的遗传毒性,本文在不同实验体系中进行了研究并获得明确的结果:在无细胞体系中,GMA与哺乳类基因组DNA发生以共价键为主要健型的结合作用,作用后的DNA或GMA的紫外吸收光谱向长波方向位移,其最大位移幅度可达20 nm,并同时有吸收强度降低。在细胞水平的实验中,观察到在低浓度下GMA即可诱发人和大鼠血淋巴细胞的DNA修复合成(UDS)及抑制S期DNA半保留复制,在一定浓度范围内这些作用呈剂量-效应关系。在动物整体实验中,观察到GMA不仅诱发雄性小鼠体内生殖细胞UDS,还引起精子畸型率升高和精子数减少。以上结果表明,GMA对人和哺乳类细胞具有明显的遗传毒性作用。

The following experiments have been investigated for evaluating the genotoxicity of glycidyl methacrylate(GMA)in the mammalian and human cells.(1)By using the absorption spectrum shift method in vitro we observed that the λ 202 nm of calf thymus DNA and the λ208 nm of GMA were shifted toward the direction of longer wave length for more than 15 nm change and the absorbance was decreased after incubation at room temperature for 15 min or more in a mixture of DNA and GMA.The results showed that the binding action between DNA and GMA occurred.The binding force is strong and not affected by sodium chloride even at high concentration and slightly decreased by 8 mol/L of urea.The binding action between DNA and GMA,therefore,might be due to the existence of a covalent bond.(2)In thecell cultures,UDS in the human and/or rat lymphocytes was induced and the DNA semiconservative replication was inhibited by GMA at a concentration of less than 5.2 mmol/L.The action showed a relationship between dose and response.(3)By using UDS and the sperm abnormality frequency tests we have studied the in vivo genotoxicity of GMA in the germ cells of the male mice.The results revealed that GMA could damage DNA,significantly increased the frequency of the sperm abnormality and reduced the number of sperms.