摘要
目的利用比较蛋白质组学的方法对胰腺癌患者血清和对照组血清例进行分析，寻找胰腺癌潜在的特异性候选标志物。方法采用双向差异凝胶电泳分离40例胰腺癌患者、10例慢性胰腺炎患者、10例良性肿瘤患者和40例健康体检者外周血清蛋白质，利用基质辅助激光解析电离飞行时间质谱技术对差异显著的蛋白进行鉴定。结果成功鉴定出2个胰腺癌差异表达蛋白：转甲状腺素蛋白和载脂蛋白E。该组蛋白在胰腺癌组中呈高表达，在正常对照组、慢性胰腺炎组和良性肿瘤组中呈低表达。结论双向差异凝胶电泳联合基质辅助激光解析电离飞行时间质谱技术筛选胰腺癌患者外周血清中的特异性生物标志物的方法具有较好的重复性和稳定性。检测鉴定的差异蛋白质载脂蛋白E、转甲状腺素蛋白可能是胰腺癌早期诊断潜在的血清特异性生物标志物。
Objective A comparative proteomic method was used to analyse serum proteins between pancreatic cancer patients and control group, and to find a new protential specific marker. Methods Comparative analysis on the pancreatic peripheral blood protein profiling from 40 pancreatic cancer patients, 10 chronic pancreatitis patients, 10 benign tumor patients and 40 cancer-free controls was carried out by 2D differential gel electrophoresis, and dif- ferentially expressed proteins were identified by matrix- assisted laser desorption/ionization time of flight mass spec- trometry. Results Two differentially expressed proteins： transthyretin and apolipoprotein E were identified. Those proteins were highly expressed in pancreatic carcinoma group compared with normal control group, chronic pancrea- tiffs group and benign tumor group. Conclusion 2D differential gel electrophoresis and matrix-assisted laser de- sorption/ionization time of flight mass spectrometry technology in screening specific serum biomarkers of pancreatic cancer has a well repeatability and stability. The identified protein transthyretin in this study may be as specific ser- um biomarkers of pancreatic carcinoma.
出处
《国际外科学杂志》
2012年第5期313-317,F0003,共6页
International Journal of Surgery
基金
2011年度安徽省科技攻关计划资助项目（NO.11010402172）
关键词
胰腺癌
蛋白质组学
双向差异凝胶电泳
基质辅助激光解析电离飞行时间质谱
标志物
Pancreatic carcinoma
Proteomics
2D differential gel electrophoresis
Matrix-assisted laser de-sorption/ionization time of flight mass spectrometry
Markers
