羊传染性脓疱病毒内蒙古分离株VIR基因的克隆与序列分析

Cloning and Sequence Analysis of VIR Gene of Inner Mongolia Isolate of Contagious Ecthyma Virus of Sheep

根据GenBank中已经发表的羊传染性脓疱病毒毒株StrainNZ2的VIR基因序列,设计合成1对引物。采用PCR技术对内蒙古分离株(OV/nm-05)的VIR基因进行特异性扩增,然后将其克隆到pEASY-T1载体中进行测序,得到该病毒的VIR基因序列。序列分析表明,OV/nm-05株与1710/03株的同源性最高,达98.0%;与513/04株的同源性最低,为95.4%。说明羊传染性脓疱病毒内蒙古分离株VIR基因与其他参考毒株之间差异不大,这为进一步从分子生物学角度了解VIR基因的结构和功能奠定了基础。

According to the published VIR gene sequence of ORFV（Strain NZ2）in the GenBank,one pair of primers were designed and synthesized.VIR gene of ORFV（OV/nm-05）of Inner Mongolia isolate were specially amplified by PCR method and then cloned into pEASY-T1 vector.The sequences of VIR gene of this virus were obtained after sequencing.The sequencing analysis showed that the homology between OV/nm-05 strain and 1710/03 strain was the highest,being 98.0%.And the homology between OV/nm-05 strain and 513/04 strain was the lowest,being 95.4%.The results demonstrated that there was a little variation between VIR gene of Inner Mongolia isolate of contagious ecthyma virus of sheep and other reference strains,which laid the foundation for further understanding the structure and function of VIR gene from the perspective of molecular biology.