利用产朊假丝酵母生产铬酵母发酵工艺的研究

Study on Fermentation Technology of Chromium-enriched Yeast Produced by Using Candida utilis

对利用产朊假丝酵母生产富铬酵母进行了研究。通过在培养基中添加Cr3+的方法制备富铬酵母,测定富铬酵母生物量及铬含量。对培养条件进行了优化,确定了最优培养条件为培养温度30℃、培养基铬添加量100μg/mL、接种量体积分数为4%～6%(V/V)、培养起始pH值为5.0、培养时间20h。在该优化条件下获得的富铬酵母生物量可达1.83g/100mL,有机铬含量可达2210μg/g。试验结果可以为富铬酵母的生产开发奠定一定的技术基础。

The fermentation technology of chromium-enriched yeast produced by using Candida utilis was studied.The chromium-enriched yeast was prepared by adding Cr3＋ in fermentation culture medium.The biomass and chromium content of chromium-enriched yeast were determined,and the fermentation culture conditons were optimized.The optimal culture conditions were as follows：the cultural temperature was 30 ℃;the the addition amount of Cr3＋ in culture medium was 100 μg/mL;the inoculation amount was 4%-6%（V/V）;the initial pH value was 5.0;and the cultural time was 20 h.Under the optimal conditions,the biomass of chromium-enriched yeast and organic chromium content were 1.83 g/100mL and 2 210 μg/g,respectively.The results lay the technology foundation for the development of chromium-enriched yeast.