不同年龄大鼠胰腺组织中胰岛素受体与磷脂酰肌醇3激酶表达水平的研究

The Expression of Rat Insulin Receptor and Phosphatidylinositol 3-kinase in Pancreas in Different Ages

目的:探讨大鼠胰腺组织中胰岛素受体与磷脂酰肌醇3激酶(PI3K)的表达随增龄的变化。方法:35只健康SD大鼠按月龄分为五组,分别为1月龄组7只,6月龄组7只,12月龄组7只,18月龄组7只,24月龄组7只。提取胰腺组织蛋白后用Western-Blot方法检测胰岛素受体和PI3K的蛋白水平,应用免疫组化方法检测胰岛素受体的分布。结果:(1)胰腺组织内胰岛素受体集中分布在胰岛细胞表面,腺泡细胞也有表达。(2)1月龄组及18月龄组大鼠胰腺组织中的胰岛素受体蛋白表达量与6月龄组比较差异有统计学意义。1月龄组PI3K蛋白质表达量与6月龄组比较差异有统计学意义。结论:进入老年期,大鼠胰腺组织中胰岛素受体及PI3K的表达升高。

Objective ：To evaluate the expression of insulin receptor and phosphatidylinositol 3-kinase （PI 3-kinase） in pancreas daring aging. Methods ：Thirty-five normal healthy Sprague-Dawley rats were divided into one month group, six months group, twelve months group, eighteen months group and twenty-four months group. Insulin receptor and PI 3-kinase of pancreas were detected by Western blot analysis. Immunohistochemistry was used to investigate the distribution of insulin receptor.Results ：Compared with the six months group, there was significant difference in insulin receptor and PI 3-kinase protein expression in one month group. Insulin receptor mainly distribute in islet, but fewer in acinar cell. Conclusion ：There is significant increase in insulin signal transduction of pancreas during aging.