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DADS诱导小细胞肺癌细胞株NCI-H446凋亡及其分子机制 被引量：1

DADS Induced Small-cell Lung Cancer Cell line NCI-H446 Apoptosis and its Molecular Mechanism
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摘要 目的探讨二烯丙基二硫(DADS)诱导人肺癌细胞株NCI-H446凋亡及其分子机制。方法流式细胞仪检测DADS诱导NCI-H446细胞凋亡率;激光共聚焦显微镜检测NCI-H446细胞内Ca2+水平变化;利用免疫细胞化学法检测NCI-H446细胞内Bcl-2蛋白表达。结果与对照组相比,DADS处理组细胞的凋亡率显著增加(P<0.05);DADS处理组细胞内Ca2+水平升高(P<0.05)。DADS下调Bcl-2(P<0.05)。结论 DADS诱导小细胞肺癌细胞株NCI-H446凋亡,其具体机制可能与DADS下调Bcl-2蛋白表达和促进细胞内Ca2+水平升高有关。 Objective To explore DADS induced small-cell lung cancer cell line NCI-H446 apoptosis and its molec- ular mechanism. Methods The apoptosis rate of NCI-H446cell line induced by DADS was analyzed using flow cytometry （FCM）;The change of Intracellular free calcium concentration was measured by laser scanning confocal microscope （LSCM）. Immunocytochemical staining was used to detect the expression of Bcl-2. Results Intracellular Ca2＋ concentration in DADS groups was higher than the control group （ P 〈 0.05 ）. DADS reduced BCL-2 expression （ P 〈 0.05 ）. Conclusions DADS could induce NCI-H446 cells apoptosis, which mechanism may be related to elevating levels of intracellular Ca2＋ and decreasing the expression of Bcl-2.
作者 童细焱 邵志滨 谢海龙
机构地区 南华大学肿瘤研究所
出处 《中南医学科学杂志》 CAS 2012年第3期227-231,共5页 Medical Science Journal of Central South China
基金 国家自然科学基金项目(81172576)资助
关键词 DADS 小细胞肺癌 BCL-2 凋亡 DADS small cell lung cancer Bcl-2 apoptosis
分类号 R730.51 [医药卫生—肿瘤] R734.2 [医药卫生—肿瘤]
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参考文献11

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中南医学科学杂志
2012年 第3期

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