Tol1转座子研究进展

Advance of Tol1 Transposable Element Study

Tol1和Tol2是在青鳉基因组中发现的具有自主活性的DNA转座子,而Tol1转座子的自主活性是新近才发现的,因此对它的报道较少。较之Tol2,Tol1可以携带更大片段的DNA进行转座,且Tol1的转座不受转座酶"过量表达抑制"的影响。研究已证实,Tol1转座子在秀丽线虫、斑马鱼、爪蟾和人等多种生物中具有转座活性。因此,在动物转基因和基因功能研究等方面有重要的应用前景。从Tol1转座子的结构特征、转座机制和作为基因转移载体的优点,以及应用研究等方面进行了简要的综述。

Toll and Tol2 are autonomous DNA-based transposable eleme,it found in medaka fish Oryzias latipes, compared with Tol2, the autonomy of Toll transposable element is identified recently, and the reports about it is limit, main advantages were that Toll was capable of carrying longer DNA fragment into chromosomes ; and the transposition of Toll was not affected by the ＂overexpression inhibition＂ of transposase. Studies have convinced that Toll was active in Caenorhabditis elegans, Danio rerio, Xenopus laevis and Homo sapiens, thus Toll was anticipated to have important applications in animal transgenic and gene functional study. The present paper gave a brief review of the structure of Tol 1 transposable element, its transposition mechanism, the advantages as gene transfer vector and applied research.