谷胱甘肽硫转移酶T1、M1、P1基因多态性与汉族人群职业性噪声聋易感性的关系

Association between Glutathione S-Transferase T1,M1,P1 Polymorphisms and Occupational Deafness among Chinese Han Population

[目的]探讨谷胱甘肽硫转移酶的3种常见基因（GSTT1、GSTM1、GSTP1）多态性与汉族人群职业性噪声聋易感性的关系。[方法]采用病例-对照研究方法,病例组为电测听结果双耳高频平均听阈≥40dB的工人161例,对照组为年龄和性别与病例匹配且电测听结果双耳高频平均听阈〈40dB的同岗位轮班工人161例,基因型的测定采用聚合酶链式反应-限制性片段长度多态性（PCR-RFLP）。[结果]通过分析发现GSTM1缺失型是职业性噪声聋的危险因素（调整OR=1.85,95%CI=1.18~2.89）,分层分析发现GSTM1缺失型与噪声作业工龄（〉20年）、噪声暴露水平[86~91dB（A）]和吸烟等危险因素结合后,存在交互作用,职业性耳聋的危险性增加（OR值变大）。[结论]GSTM1缺失型可能是汉族人群职业性噪声聋的危险因素之一,而且可能与暴露水平、吸烟因素有交互增强作用。

[ Objective ] To investigate whether any of the three functional glutathione S-transferase （GSTT1, GSTM1, GSTPI） polymorphisms are associated with the susceptibility to occupational noise-induced deafness in Chinese Han population. [ Methods ] A case-control study was conducted： 161 cases whose average hearing threshold were no less than 40dB in high frequency, and 161 controls in the same working position with the cases which were matched with age and gender. Polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） was used as genotyping. [ Results 3 GSTM1 null genotype was a risk factor for occupational noise-induced deafness （adjusted OR=1.85, 95%CI=1.18-2.89）. When combined with noise exposure years （〉20 years）, noise exposure levels [ 86-91dB（A） ] and smoking, the risk became much higher （OR increased）. [ Conclusion ] GSTM1 null genotype may be a risk factor for occupational noise-induced deafness in Chinese Han population. It may have interactions with exposure level and smoking status.