高效液相色谱法测定肌氨肽苷注射液中次黄嘌呤的含量

Determination of Hypoxanthine in Jiantaigan Injection by HPLC

目的:建立高效液相色谱法测定肌氨肽苷注射液中次黄嘌呤的含量。方法:以十八烷基硅烷键合硅胶为填充剂,磷酸二氢钾(0.01mol/L)-甲醇(100:0.2)为流动相,检测波长260nm。结果:平均回收率为100.56%,RSD=1.57%。结论:该方法简便、准确。

Objective：To establish a method of hypoxanthine for Jiantaigan injection by high-performance liquid chromatography.Methods：The method uses a C18 column,potassium dihydrogen phosphate（0.01mol/L）-methanol（100：0.2）as the mobile phase.The detection wavelength was set at 260nm.Results：The average recovery was 100.56%,RSD was 1.57%.Conclusion：The method is simple and accurate.