摘要
目的:探讨曲安奈德对核因子-κB(NF-κB)及血管内皮生长因子(VEGF)在大鼠视网膜新生血管病变中表达的影响。方法:选择7 d SD幼鼠40只,其中30只大鼠建立氧诱导的视网膜新生血管病变模型,10只置于正常环境中。30只大鼠中20只大鼠出氧箱后给予玻璃体腔内注射曲安奈德1μl(0.04 mg),对侧眼注射平衡盐溶液(balanced saltsolution,BSS)作为对照,分为高氧+药物治疗组和高氧+BSS液对照组,每组20只眼;另10只大鼠作为高氧对照组。视网膜组织切片HE染色,免疫组织化学法检测NF-κB及VEGF的表达。结果:高氧+药物治疗组与高氧对照组及高氧+BSS组相比,新生血管内皮细胞核数目明显减少(P<0.01),高氧+药物治疗组的NF-κB及VEGF的表达均低于高氧对照组及高氧+BSS组(P<0.05)。VEGF表达升高的同时,NF-κB的表达呈升高趋势。结论:曲安奈德明显抑制NF-κB及VEGF的表达,即有抑制新生血管形成的作用,NF-κB与VEGF的表达呈正相关,在新生血管形成过程中起协同作用。
Objective：To investigate the effect of triamcinolone acetonide on the nuclear factor-KB （NF-kB） and vascular endothelial growth factor（VEGF） expression in retinal neovascularization of rats. Methods：Forty SD 7 days old rats were selected, 10 rats were in normal environment as control. Models of retinal neovascularization of 30 rats were established using oxygen-induced, which were divided into other three groups, 10 rats cavum vitreum were injected with 1ul （0.04 mg） of triamcinolone acetonide as hyperoxia combined with medication group, 10 rats cavum vitreum were injected with balanced saltsolution（BSS） as hyperoxia combined with BSS group and the left 10 rats acted as hyperoxia control group. HE staining, the expression of NF-KB and VEGF using immunohistochemical method,in the retina of 20 eyes of each group were explored. Results： The number of neovascular nuclei of the rat retina of hyperoxia combined with medication group were significantly less than that of hyperoxia control group and hyperoxia combined with BSS group （ P 〈 0.01 ）. However, the expression of NF-KB and VEGF were significantly lower （ P 〈 O. 05 ）. With the high level of expression of VEGF,the expression of NF-KB in the retina was also rising. Conclusions： Triamcinolone plays a role in inhibiting retinal neovascularization and the expression of NF-KB and VEGF,and the expression of NF-KB and VEGF in the retina showes positive correlation.
出处
《蚌埠医学院学报》
CAS
2012年第5期512-515,共4页
Journal of Bengbu Medical College
基金
蚌埠医学院科研基金资助项目(BY0529)
