高效液相色谱法测定大鼠血浆中凡德他尼的浓度

Determination of vandetanib in rat plasma by high performance liquid chromatography

目的 建立大鼠血浆中凡德他尼的高效液相色谱测定方法,为凡德他尼药代动力学研究提供参考.方法 血浆样品经处理后,采用高效液相色谱法进行分析.Agilent HC-C18柱分离,流动相采用乙腈-0.02 mol/L磷酸二氢钠溶液（80∶20）,流速为1 ml/min；利用光电二极管阵列检测器对流份进行检测,柱温30℃.结果 内源性物质不干扰测定,凡德他尼的线性范围为0.5 ～ 10 mg/L（R =0.997）,最低定量限值为0.5 mg/L,基质效应为95.2％～103.7％,加样回收率为73.1％ ～87.9％.结论 建立的高效液相色谱方法简便、灵敏、准确、所需样本量小,可为凡德他尼血药浓度检测提供依据.

Objective To develop a high performance liquid chromatography（HPLC）method for the deter- mination of vandetanib in rat plasma. Methods The plasma samples were extracted and separated by an Agilent HC-C18 column, using methanol-0.02 mol/L sodium dihydrogen phosphate （80：20 ）as the mobile phase. The flow rate was 1.0 ml/min. The fractions were simuhaneously detected by DAD with wavelength at 270 nm. Results The method was linear for vandetanib in the range of 0. 5-10 mg/L. The correlation coefficient was 0. 997 and the lower limit of quantification was 0. 5 mg/L. The matrix effect was from 95.2% to 103.7%. The extraction recovery was in the range of 73.1%-87.9%. Conclusions The HPLC method is simple, sensitive and accurate. It is suitable for the determination of vandetanih in rat plasma.