乌鲁木齐市水磨沟区霍乱弧菌分离株脉冲场凝胶电泳分析

Pulsed-field gel electrophoresis of Vibrio cholerae isolates from the Shuimogou District of the City of Urumqi,Xinjiang Uygur Autonomous Region

目的了解新疆乌鲁木齐市水磨沟区相关年份霍乱弧菌分离株分子分型特征和遗传相关性。方法用脉冲场凝胶电泳(PFGE)对霍乱菌株进行分子分型,用BioNumericsV4.0软件UPGMA方法进行聚类分析。结果 54株受试菌以NotⅠ酶切后分为34个型,除1998023、2001009、2006010、1998160外,其他菌株带型高度相似(相似系数0.98),不同年份分离菌株型别不相同。结论乌鲁木齐水磨沟区霍乱弧菌可能含有多个克隆系,且分离出的霍乱弧菌为地方株。

Objective To ascertain the molecular and genetic correlation between characteristics of Vibrio cholerae isolates from Shuimogou,City Urumqi,Xinjiang in different years.Methods Pulsed-field gel electrophoresis（PFGE） was used to molecularly type cholera strains,and cluster analysis was performed with UPGMA using BioNumericsV4.0 software.Results Fifty-four strains were tested;Not Ⅰ digestion resulted in 34 types that were highly similar（similarity coefficient of 0.98） with the exception of 1998023,2001009,2006010,and 1998160;isolates from different years were of dissimilar types.Conclusion Vibrio cholerae from the Shuimogou District of Urumqi may consist of multiple clones,and Vibrio cholerae isolates may represent local strains.