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红细胞膜变形能力与胞内血红蛋白聚合状态及细胞形态的关系 被引量：1

Study on the correlation of membrane deformability and intracellular hemoglobin aggregate as well as cell morphology
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摘要 以显微激光散射光谱和图像分析技术，对单个活态红细胞在无扰、在位、实时的情况下，测量了人血红细胞胞膜的变形能力。 The membrane deformability and the intracellular hemogdob in aggregate as well as cell morphology were measured by a microscope laser light scattering spectroscopy and image analyzing system on single intact red blood cell. Their correlation with physiological conditions was also investigated.
作者 黄耀熊 李平阳 谭润初 李永龙 贺奇才
机构地区 暨南大学生物医学工程研究所 中山医科大学物理教研组
出处 《暨南大学学报（自然科学与医学版）》 CAS CSCD 2000年第1期37-41,共5页 Journal of Jinan University(Natural Science & Medicine Edition)
基金 国务院侨办重点攻关项目基金!9803139 广东自然科学基金!9803162
关键词 血红蛋白 聚合状态 细胞形态 红细胞膜 变形能力 membrane flexibility intracellular hemoglobin aggregate cell morphology microscope laser light scattering spectroscopy and image analyzing system red blood cell
分类号 R318.01 [医药卫生—生物医学工程] Q24 [生物学—细胞生物学]
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  • 1[1]Smith JA, Matin DT, Telford RD, et al. Greater erythrocyte deformbility in world-class endurance athlete [J]. Am J Physical,1999 ;276(6) :2188-93.
  • 2[3]Jordan J, Kiernan W, Merker H J, et al. Red cell membrane skeletal changes in marathon runners [J]. Int J Sports Med, 1998, 19:16219.
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  • 4[8]Kuzman D, Znidarcic T, Gros M, et al. Effect of pH on red blood cell deformability[J]. Pflugers Arch, 2000, 440: R193-194.
  • 5[9]Igliè A, H, gerstrand H, Kralj-Igliè V, et al. A possible physical mechanism of red blood cell vesiculation obtained by incubation at high pH[J]. J Biomechanics, 1998, 31:151-156.
  • 6[10]Salhany J M, Cordes K A, Sloan R L. Characterization of the pH dependence of hemoglobin binding to band 3 : evidence for a pH-dependent conformational change within the hemoglobin-band 3 complex[J]. Biochim Biophys Acta, 1998,1371 : 107-113.
  • 7[11]Ivanov I T. Low pH-induced hemolysis of erythrocytes is related to the entry of the acid into cytosole and oxidative stress on cellular membranes[J]. Biochim Biophys Acta, 1999, 1415:349-360.
  • 8[12]Park S, Kose T, Hamasaki M, et al. Effects of the pH-controlled hemoglobin vesicles by CO2 gas[J]. Artif Cells Blood Substit Immobil Biotechnol, 1998, 26(5-6): 497-506.
  • 9[13]Nielsen HB, Madsen P, Svendsen LB, Roach RC, Secher NH. The influence of PaO2 pH and SaO2 on maximal oxygen uptake[J]. Acta Physiol Scand, 1998, 164(1) :89-7.
  • 10[14]Nageswari K, Banerjee R, Gupte RV, et al. Effects of exercise on reheological and microcirulatory parameters [M]. Clin Hemorheol Microcir, 2000, 23 (2-4): 243-247.

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暨南大学学报（自然科学与医学版）
2000年 第1期

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