组培甜菊糖苷的变化研究

The Study of Stevia glucoside Change in Stevia Tissue Culture

研究了甜菊组培过程中 ,甜菊苷 (Stevioside,缩写为 SS)和丽鲍迪苷 A(Rebaudioside,缩写为RA)两种主要甜味成份的变化以及不同浓度的 6 -卞氨基腺苷 (6 - BAP)对愈伤组织合成 SS及 RA的影响 ,并初步探讨了根部在糖苷合成过程中的作用 .结果表明 :甜菊愈伤组织合成糖苷的能力随培养时间的不同而异 .培养 10 d的愈伤组织可检测到少量残余叶片的 SS及 RA,而培养 2 0 d,30 d的愈伤组织则未检出 .培养 4 0 d的愈伤组织仅检测到 RA,表明此时的愈伤组织又恢复了合成糖苷的能力 ,推测与叶绿体的再分化有关 .在含有不同浓度 6 - BAP的培养基中培养 2个月的愈伤组织都能够合成糖苷 ,但所合成糖苷的组分不同 .其中 ,在含有 0 .5mg/L,1mg/L,2 mg/L 6 - BAP培养基中的愈伤组织只能积累 RA,且随浓度的增加 ,RA的含量逐渐降低 ;而含有 3mg/L 6 - BAP培养基中的愈伤组织只能合成 SS.有根苗可合成 SS及 RA.而无根腋芽苗只能合成

The changes of two main sweet content, Stevioside(SS) and Rebaudioside (RA),the effects of 6 benzylaminopurine riboside(6 BAP) on the two sweet content in callus of stevia rebaudiana, as well as the role of root in the process of stevioside biosynthesis have been studied.the result showed that the ability for stevioside biosynthesis of callus altered with the lapse of cultural time; when cultured 10 days, Trace SS and RA were tested on callus. 20、30 days later,neither SS nor RA were tested, which indicated the callus had no ability for stevioside biosynthesis.But 40 days later,only RA was synthetized. It seems that the stevioside biosynthesis is related to re differentiation of chloroplast.when cultured 60 days later,all the callus with different concentration 6 BAP do have ability to synthetize SS or RA.The callus in culture with 0.5 mg/L,1 mg/L, 2 mg/L 6 BAP can only synthetize RA, while the callus with 3mg/L 6 BAP only sythetize SS.The rooted seedling demonstrated the ability to synthetize both SS and RA,Shooted seedling only to synthetize SS.