人参锈腐病菌双胞柱孢的鉴定及其生物转化功能研究

Identification of Ginseng Root Rust-rot Pathogen Cylindrocarpon didymium and Its Biotransformation of Ginsenosides

从采自辽宁省桓仁县患有锈腐病的人参组织中分离得到多株柱孢属真菌。通过柯赫氏法则证明柱孢属真菌对人参具有致病性,可引起典型人参锈腐病。经形态学鉴定,确定致病真菌SR79、SR86、SR88、SR90、SR91、SR94、SR95、SR96为双胞柱孢Cylindrocarpon didymium。进一步研究这些菌株对人参皂苷的生物转化作用,通过TLC和HPLC检测,证明菌株SR79具有较强的转化活性,能够将人参皂苷Rb1和人参皂苷Rd转化成稀有人参皂苷Rh2。结果表明,人参锈腐病菌SR79菌株具有人参皂苷生物转化开发潜力。

Several Cylindrocarpon strains were obtained from rust rot disease -suspected roots of ginseg grown in Huanren areas of Liaoning province. They were confirned to be of pathogenicity to ginseng roots according to Koch ＇s postulation. The pathogenic strains SR79, SR86, SR88, SR90,SR91, SR94, SR95, SR96 were morphologically identified as Cylindrocarpon didymium. Among these pathogens strain SR79 was found to show the specificity to transform Rb1 and Rd ginsenosides into ginsenoside Rh2 by methods of TLC and HPLC. This strain was shown to possess commercial potential in ginsenoside transformation.