CD4^+ CD25^+ Foxp3^+调节性T细胞在成人过敏性哮喘急性发作期和缓解期外周血中的表达

The Level of CD4^+ CD25^+ Foxp3^+ Tregs in Peripheral Blood of Allergic Asthma Patients with Acute Stage or Stationary Phase

目的:探讨成人过敏性哮喘急性发作期和缓解期外周血中CD4+CD25+Foxp3+调节性T细胞的表达情况及与病情的相关性。方法:收集健康人、过敏性哮喘急性发作期和缓解期患者外周血,流式细胞术测定CD4+CD25+Foxp3+Treg的比例。结果:CD4+CD25+Foxp3+Treg细胞在三组间的差异有统计学意义。哮喘缓解期组、哮喘急性期组较健康对照组该细胞比例均明显下降,哮喘急性期组较哮喘缓解期组更低。CD4+CD25+Foxp3+Treg细胞数量与哮喘病情严重程度呈负相关。结论:CD4+CD25+Foxp3+调节性T细胞可能在过敏性哮喘的发病过程中起重要作用,它的降低导致的免疫缺陷可能与哮喘的发生相关。

Objective： To investigate the levels of CD4＋ CD25＋ Foxp3＋ Tregs in peripheral blood of allergic asthma patients with acute stage or stationary phase. Methods： Peripheral blood was collected from healthy or asthma patients with acute stage or stationary phase. The percentage of CD4＋^CD25＋^Foxp3^＋ Tregs was detected by flow cytometry. Results： There were statistical differences between three groups. Significant decrease of the percentage of CD4＋^CD25＋^Foxp3^＋ Tregs in peripheral blood was observed in acute stage group and stationary phase group compared with control group（P〈0.05）. This was also found between acute stage group and stationary phase group（P〈0.05）. There was negative correlation between the count of CD4＋^CD25＋^Foxp3^＋ Tregs and the severity of asthma. Conclusion： The CD4＋^CD25＋^Foxp3^＋ Tregs may play key role in the pathogenesis of asthma and its decrease, which leads to immune dysfunction, may involve in the mechanisms of asthma.