硫化氢对海洛因依赖大鼠一氧化氮/一氧化氮合酶体系的影响

The effect of hydrogen sulfide on nitric oxide /nitric oxide synthase system in heroin dependent rats

目的探讨硫化氢(H2S)对海洛因依赖大鼠一氧化氮(NO)/一氧化氮合酶(NOS)体系的影响。方法实验大鼠随机分成3组:正常对照组、海洛因依赖组(herion组)、海洛因依赖+NaHS组(heroin+NaHS组)。采用比色法测定大鼠血浆及海马组织NO含量,Real time PCR测定海马组织nNOS mRNA表达量。结果血浆及海马NO含量比较,herion组与heroin+NaHS组均高于对照组(<0.05),heroin+NaHS组低于herion组(<0.05);海马组织nNOS mRNA表达量比较,herion组高于对照组(<0.05),heroin+NaHS组与对照组无显著差异(>0.05),heroin+NaHS组低于herion组(<0.05)。结论海洛因依赖可导致大鼠体内NO水平升高,H2S供体NaHS可抑制NO的产生,下调nNOS mRNA表达。

Objective To explore the effect of hydrogen sulfide （H2S） on nitric oxide （NO） /nitric oxide synthase （NOS） system in heroin dependent rats. Methods Experimental rats were divided into three groups： control group, heroin group and heroin ＋ NariS group. The level of NO in plasma and hippocampus was detected by the spectrophotometer method. The expression ofrtNOS mRNA was observed by Real time PCR. Results Compared with control group, NO in plasma and hip- pocampus increased （P 〈 0.05） in heroin group and heroin＋NariS group, the expression ofnNOS mRNA up-regulated （P 〈 0.05） in heroin group. Compared with heroin group, NO in plasma and hippocampus decreased （P 〈 0.05） and the expression ofnNOS mRNA down-regulated （P 〈 0.05） in heroin＋NariS group. Conclusion Heroin dependence can increase NO level and up-regulate the expression ofnNOS mRNA in rats. Exogenous H：S donor NariS can inhibit the production of NO and down-regulate the expression ofnNOS mRNA.