Sus scrofa interferon epsilon-1基因的克隆与序列分析

Cloning and Sequence Analysis of Sus scrofa interferon epsilon-1 Gene

[目的]克隆分析Sus scrofa interferon epsilon-1基因,以期为其生物学功能研究奠定基础。[方法]利用Homo sapiens interferon epsi-lon 1(IFNE1)序列(NM_176891.3)对猪HTG库进行搜索,通过对获得的2个片断(CU074336、AC127471)的序列分析,在5'-UTR和3'-UTR设计1对克隆引物,对7日龄仔猪的胃组织进行RT-PCR,将PCR产物克隆、测序,并进行相关分析。[结果]同源性分析结果表明,猪SIFNE1与人、小鼠interferon epsilon-1基因cDNA编码区(CDS)的同源性分别为83.6%和69.2%;蛋白序列同源性分别为76.2%和55.2%。推测其氨基酸序列信号肽为第1～21位氨基酸,IFabd结构域为第59～176位氨基酸,结构特征与人、小鼠的interferon epsi-lon-1相一致。[结论]该研究克隆了Sus scrofa interferon epsilon-1基因,为进一步研究SIFNE1基因的生物学功能奠定了基础。

[Objective] To clone and analyze the Sus scrofa interferon epsilon-1 gene,so as to lay foundation for the study of its biological functions.[Method] The swine HTG database was searched with the Homo sapiens interferon epsilon-1（IFNE1） sequence（NM_176891.3）,two fragments（CU074336 and AC127471） were obtained and analyzed by sequencing.One pair of primers was cloned at 5′-UTR and 3′-UTR to analyze the gastric tissue of seven-day-old piglets by RT-PCR,the PCR products were cloned,sequenced and correlation analyzed.[Result] The homology analysis showed that the homology of swine SIFNE1 with human and mice interferon epsilon-1 gene cDNA CDS was 83.6% and 69.2% respectively,the homology between their protein sequences was 76.2% and 55.2% respectively.The signal peptide of amino acid sequence was predicted to be the 1-12 position of amino acid,the protein domains of IFabd were the 59-176 position of amino acid,which was consistent with the interferon epsilon-1 of human and mice.[Conclusion] The study cloned the Sus scrofa interferon epsilon-1 gene,and laid foundation for furthur studying its biological functions.