基因组重排在产纤维素酶木霉改造中的应用

Application of Genome Shuffling in the Breeding of a Cellulase-producing Trichoderma

[目的]探讨基因组重排技术在提高木霉纤维素酶活力育种的应用可行性。[方法]利用基因组重排的方法,对一株产纤维素酶的木霉进行改造,并对改造后菌株的纤维素酶活力进行测定和比较。[结果]数据表明诱变育种后纤维素酶活力是野生型菌株的1.17倍;第一轮基因组重排后融合子的平均纤维素酶活力是野生型菌株的1.82倍;第二轮重排后融合子的平均纤维素酶活力是野生型菌株的3.27倍。[结论]该研究表明,基因组重排可以在较短时间内实现微生物某些特性的大幅度提高。

[Objective] To explore the feasibility of genome shuffling to improve the ability of Trichoderma on degradation of cellulose.[Method] The method of genome shuffling was used to transform a cellulase-producing Trichoderma,and determine and compare its cellulose activity.[Result] The cellulose activity after mutation breeding was 1.17 times that of wild strains,the average cellulase activity of fusants was 1.82 times that of wild strains after the first round of genome shuffling,and 3.27 times after the second round.[Conclusion] Genome shuffling can effectively improve the certain characteristics of microorganisms in a relatively short period.