流感病毒快速诊断试剂中HA抗原热稳定性的研究

Study on the Heat Stability of HA Antigen in Influenza Virus Rapid Diagnosis Kit

[目的]筛选流感病毒HA抗原保护剂。[方法]将H3N2和H1N1亚型流感病毒分别加入A～F组中,在37℃下进行加速试验,并通过血凝试验测定HA滴度。[结果]在28 d A组(添加PBS缓冲液)中H3N2和H1N1亚型流感病毒的HA抗原血凝滴度分别为20和32。F组(添加BSA、岩藻糖、Proclin 300、Triton 100)HA抗原的热稳定性最好,在28 d H3N2和H1N1亚型流感病毒HA抗原血凝滴度分别为48和96。[结论]F组成分对流感病毒HA抗原的保护作用最好,可作为保护剂候选配方。

[Objective] The research aimed to screen out the protective agent of influenza virus.[Method] H3N2 and H1N1 subtypes of influenza virus were added to A-F groups to make the accelerated test at 37 ℃.And HA titer was test by hemagglutination test.[Result] HA antigen hemagglutination titer of H3N2 and H1N1 subtype of influenza virus in A group（adding PBS buffer）were 20 and 32 respectively after 28 days.The heat stability of HA antigen in F group（adding BSA,fucose,Proclin 300,Triton 100）were the best and HA hemagglutination titer of H3N2 and H1N1 subtype of influenza virus were 48 and 96 respectively after 28 days.[Conclusion] The components in F group had the best protective effects on HA antigen of influenza virus and could be used as the candidate agent of protective agent.