双氢杨梅素在家兔体内的药动学研究

Study on the Pharmacokinetics of Dihydromyricetin in Rabbits

[目的]研究双氢杨梅素在家兔体内的药动学特征。[方法]经单剂口服给药后,收集家兔血样,经离心后,采用紫外分光光度法于280 nm波长处进行血药浓度测定。[结果]双氢杨梅素的血药浓度—时间曲线符合一级消除的单室模型,Ke为(0.76±0.13)h-1,Ka为(0.85±0.24)h-1,t1/2(Ka)为(0.82±0.31)h,t 1/2(Ke)为(0.91±0.44)h,Tmax为(1.5±0.46)h,Cmax为(12.36±2.7)mg/L,AUC0→∞为(45.45±22.3)mg.h/L,CL/F为(4.71±1.21)L/(h.kg),V/F为(6.17±3.12)L/kg。[结论]该方法简便、可靠、灵敏,适用于双氢杨梅素的药动学研究。

[Objective] To study the pharmacokinetics of dihydromyricetin in rabbits.[Method] After oral administration with dihydromyricetin at a single dose,the blood samples of rabbits were collected and centrifuged.Dihydromyricetin concentration was determined with UV spectrophotometry at the wavelength of 280 nm.[Result] The concentration-time curve of dihydromyricetin was described by a single compartment model,Ke was（0.76±0.13）h-1,Ka was（0.85 ± 0.24）h-1,t1/2（Ka） was（0.82±0.31）h,t1/2（Ke） was（0.91 ± 0.44）h,Tmax was（1.5 ± 0.46）h,Cmax was（12.36±2.7）mg/L,AUC0→∞ was（45.45 ± 22.3）mg·h/L,CL/F was（4.71±1.21）L/（h·kg）,V/F was（6.17±3.12）L/kg.[Conclusion] The method is simple,reliable and sensitive and can be used for the pharmacokinetic research of dihydromyricetin.