海洋假单胞杆菌HW08基因组文库的构建及序列功能分析

Construction of Genomic Library for Marine Pseudomonas HW08 and Analysis of Its Sequence Function

通过超声波处理HW08基因组,将获得的1～2 KB DNA片段与pUC18质粒进行连接后转化,构建文库。随机筛选200个克隆进行序列测定,快速分析了海洋假单胞杆菌HW08基因组。结果表明:构建了HW08菌株的基因组部分文库,获得序列173个,包含7个载体序列和7个重叠群,其中最长重叠群为重叠群4,包含3段基因序列,分别为Chemotaxis Protein、Hypotheticalprotein和LacI family transcription regulator。重叠群3为DNA修复蛋白,其最大相似性为83%。166个序列中酶类占绝大部分,同时也包含37个新基因。

A 1~2 kb DNA fragment of HW08 genome was obtained by ultrasonic treatment,and the genomic library of HW08 was constructed after connecting the pUC18 plasmid with the obtained fragment and transformation.The random selected 200 clones were sequenced and analyzed in order to quick analyze the genome of marine pseudomonas HW08.The results showed that a partial genomic library of HW08 was constructed,and the obtained 173 sequences have seven vector sequences and seven contigs,in which the longest contig is the fourth contig which contained Chemotaxis Protein,Hypothetical protein and LacI family transcription regulator.The third contig is DNA repair protein,and its maximum similarity is 83%.Among the 166 sequences,the enzyme occupied most of the part;meanwhile 37 new genes were also included.