不同生长年限人参中8种酶活力比较

Activity comparison of eight kinds of enzyme in ginseng from different growth term

目的:通过对4、5、6、7年生人参中的8种酶活力进行比较,为人参的生长发育研究奠定基础。方法:采用中性缓冲液提取粗酶液,应用分光光度法对4个不同生长年限的人参中苹果酸脱氢酶(MDH),乳酸脱氢酶(LDH),乙醇脱氢酶(ADH),葡萄糖-6-磷酸脱氢酶(G6PDH),超氧化物歧化酶(SOD),葡萄糖磷酸异构酶(GPI),谷丙转氨酶(GPT)和延胡索酸酶(Fumarase)8种酶活力进行比较。结果:除SOD和GPI外,其他6种酶活力均是6年生人参样品最高,4年生样品活力最低。结论:这8种酶的活力可以作为不同生长年限人参生长发育研究的理论依据。

Objective： To comparison the activities of eight enzymes in Ginseng which are from 4 year, 5 year, 6 year, 7 year, in order to provide the foundation of the growth and development of ginseng. Methods： Adopt neutral buffer solution to extract the enzyme solution of Ginseng. Use spectrophotometry to test the activities of Malate Dehydrogenase （MDH）, Lactate Dehydrogenase （LDH）, Alcohol Dehydrogenase （ADH）, Glucose 6-phosphate Dehydrogenase （G6PDH）, Superoxide dismutase （SOD）, Glucose Phosphate Isomerase （GPI） Glutamic-pyruvic transaminase （GPT）, and Fumarase. Results： In addition to superoxide dismutase （SOD） and glucose phosphate isomerase outside, six other kinds of enzyme activities which are from Ginseng of 6 years are the highest, while that of 4 years Ginseng are the lowest. Conclusion： These 8 kinds of the enzyme activities can be used as growth theoretical basis of different growth year Ginseng.