不同产地夏枯草中抗非小细胞肺癌成分科罗索酸的差异比较

Comparision of anti-non small cell lung cancer compound corosolic acid in Prunella vulgaris L.from different regions

目的:比较不同产地夏枯草中抗非小细胞肺癌活性成分科罗索酸的含量差异。方法:通过四甲基偶氮唑蓝(MTT)和吖啶橙(AO)/溴化乙啶(EB),评价科罗索酸对非小细胞肺癌细胞的增殖抑制以及凋亡作用;建立高效液相色谱法,比较14个产地夏枯草中科罗索酸的含量差异。结果:科罗索酸抑制SPC-A-1人非小细胞肺癌的生长增殖(IC50=1.05×10-6mol/L),并且诱导SPC-A-1细胞凋亡。14个产地夏枯草中科罗索酸的含量差异较大,山西产地的含量最高,河南-2产地的含量最低。结论:科罗索酸为夏枯草中的抗非小细胞肺癌的活性成分,不同产地夏枯草的科罗索酸含量差异较大。

Objective： To compare the content difference of anti-non small cell lung cancer active ingredient corosolic acid in Prunella vulgaris L. from different regions. Methods： MTT and acridine orange （AO）-ethidium bromide （EB） methods were established to evaluate anti-proliferation and apoptosis activity. A high performance liquid chromatography method was established for this comparision. Results： Corosolic acid inhibited SPC-A-1 cell proliferation and IC50 was 1.05x 10-tmol/L. Corosolic acid also induced SPC-A-I cell apoptosis. There was obvious differences on the content of corosolic acid in Prunella vulgaris L. from 14 different regions. Shanxi had the highest content of corosolic acid while Henan-2 had the lowest. Conclusion： Corosolic acid was a anti-non small cell lung cancer active ingredient in Prunella vulgaris L. The content of corosolic acid in Prunella vulgaris L. from different regions has a significant difference on the market.