肾系膜细胞在补肾药物基础上进一步上调成骨细胞collagenⅠ和cbfα1基因表达

Effects of mesangial cells on expressions of collagen Ⅰ and cbfα1 in osteoblasts base on the foundation of tonifying kidney herbs

目的:观察系膜细胞是否影响补肾药物对成骨细胞的作用。方法:空白组大鼠灌服蒸馏水,药物组大鼠以熟地鳖甲1:1配比制成浸膏,3.3g浸膏/kg体质量/d灌服,3d后取血离心,获得无药血清和熟地鳖甲含药血清。无药血清、熟地黄鳖甲含药血清及肾系膜细胞处理的熟地鳖甲含药血清作用于成骨细胞3d,MTT法观察成骨细胞增殖率,实时荧光定量PCR检测成骨细胞Ⅰ型胶原(collagenⅠ)和核心结合因子α1(cbfα1)基因表达。结果:熟地黄、鳖甲含药血清可促进成骨细胞增殖,经肾系膜细胞处理后的含药血清进一步促进细胞增殖(P<0.05),荧光定量PCR显示含药血清组成骨细胞collagenⅠ和cbfα1基因表达量明显上调(P<0.05),经肾系膜细胞处理后的含药血清2种基因表达量进一步增加(P<0.05)。结论:肾脏固有细胞——肾系膜细胞可在补肾药物基础上进一步促进成骨细胞的增殖和成骨相关基因的表达。

Objective： Tonifying kidney herbs can promote the proliferation, differentiation and other functions of osteoblast. There is a intimate connection between kidney and bone. Renal mesangial cells may secrete some factors effecting osteoblast. Aim of our study is to investigate the effects of mesangial cells on osteoblast which have been regulated by tonifying kidney herbs. Methods： Rat were divided into 2 groups： control group, drenched with distilled Water; Radix Rehmanniae Praeparata （RRP） Carapax Trionycis （CT） group, with RRP CT extractum, 3.3g/kg/d, for 3 days, when non-herb and RRP CT serum obtained.Osteoblasts were treated respectively with non-herb serum, contained herbs serum and contained herbs serum processed by mesangial ceils for 3 days. MTT method was used to investigate proliferation rate of osteoblasts. Expressions of collagen I and cbfctl were examined using real-time fluorescence quantity assay.Results： Contained Rehmannia glutinosa and Turtle shell serum promoted the proliferation of osteoblasts, while contained herbs serum processed by mesangial cells got further effects（P〈0.05）. Results of real-time fluorescence quantity assay showed that expressions of collagen I and cbfal were upregulated in contained herbs serum group（P〈0.05） and the effects were reinforced by mesangial cells （P〈0.05）. Conclusion： Mesangial cells can further promote proliferation of osteoblasts and expressions of related genes.