栀子与茵陈蒿汤对非酒精性脂肪性肝病大鼠脂质代谢及血清酶学影响的比较

Comparison of the effects of Yinchenhao Decoction and gardenia on the lipid metabolism and serum enzymology in rats with non-alcohol fatty liver

目的:观察比较栀子与茵陈蒿汤对非酒精性脂肪性肝病大鼠脂质代谢及血清酶学的影响。方法:用高脂饮食建立大鼠非酒精性脂肪肝模型,自造模第6周起,28只大鼠被随机分为模型组、栀子组、茵陈蒿汤组和易善复组,每组7只,灌胃饮用水或给药5周。通过肝脏组织HE染色观察肝组织病理学;肝功酶试剂盒及血脂试剂盒检测大鼠血清生化指标及肝组织甘油三酯(TG)含量。结果:模型组大鼠肝脏存在明显脂肪变性,并可见炎症细胞浸润,肝组织TG、血清总胆固醇(TC)、甘油三酯(TG)含量、血清丙氨酸氨基转移酶(ALT)、门冬氨酸氨基转移酶(AST)活性均显著升高(P<0.01),血清高密度脂蛋白胆固醇(HDL-C)含量显著降低(P<0.01);栀子组、茵陈蒿汤组大鼠肝脂肪变性及炎症反应较模型组明显减轻,肝组织TG、血清TG、TC含量、血清ALT、AST活性均较模型组显著降低(P<0.05,P<0.01),血清HDL-C则显著升高(P<0.01);栀子组在降低血清ALT及血清TC含量方面优于茵陈蒿汤组(P<0.05,P<0.01)。结论:栀子与茵陈蒿汤均能明显改善脂肪肝大鼠肝组织病理学变化,对非酒精性脂肪性肝病有较好的干预作用,而栀子在改善胆固醇代谢及抗炎症损伤方面,效果优于茵陈蒿汤。

Objective： To observe and compare the effects of Yinchenhao Decoction （Decoction for lipid-lowering and recovery of hepatic function） and gardenia on the lipid metabolism and serum enzymology in rats with non-alcohol fatty liver. Methods： Rat models with non-alcohol fatty liver established by feeding with high fat food. Since the sixth week, 28 rats were randomly divided into 4 groups. They were the Model group, gardenia group, Yinchenhao Decoction group and the polyene phosphatidylcholine capsules group. Each of them had 7 rats. They were incubated with drinking water or the medicine for 5 weeks. Then, the pathological examination of liver tissues was performed under a microscope by HE staining, the Hepatic tissue TG and biochemical indices of liver function and blood lipid were measured. Results ： The livers of the model group existed obvious fatty denaturation, and they also have inflammatory cell infiltration. The hepatic tissue TG, serum total cholesterol （TC）, tralyceride （TG）, serum levels of alanine transaminase （ALT）, aspartic transaminase （AST） increased in model group （P〈0.01）, the high density lipoprotein cholesterol （HDL-C） decreased remarkable （P〈0.01）. The liver fatty denaturation in Yinchenhao Decoction group and gardenia group reduced than that in model group, and the hepatic tissue TG, TC, TG, serum levels of ALT, AST decreased in Yinchenhao Decoction group and Gardenia group （P〈0.05, P〈0.01） as compared with those of the model group, and HDL-C increased remarkable （P〈0.01）. Conclusion： Yincbenhao Decoction and gardenia are effective in improving the pathological changes and the pathological changes in hepatic tissues. And they all have the intervening effect on the nonalcoholic fatty liver disease （NAFLD） model in rat, moreover, gardenia is better than Yinchenhao Decoction in cholesterol metabolism and the risistance in inflammation lesions.