梅术溃结灌肠汤对溃疡性结肠炎大鼠白介素-6、白介素-10及丙二醛的影响

Effects of Mei colitis and enema soup on interleukin-6,interleukin-10 and malondialdehyde of ulcerative colitis rats

目的:探讨梅术溃结汤灌肠治疗溃疡性结肠炎的部分作用机制。方法:实验大鼠随机分为空白组,模型组,药物低、中、高剂量组5组,每组10只;每日灌肠1次,共4周。放射免疫法测血清IL-6,IL-10,比色法测MDA。结果:模型组与空白组比较,IL-6、IL-10、MDA水平有统计学差异(P<0.01)。药物组IL-6、IL-10、MDA含量与模型组差异有统计学意义(P<0.01);IL-6高剂量组高于低剂量组、MDA低剂量组大于高剂量组,差异有统计学意义(P<0.05),MDA与剂量的增加呈负相关;IL-10与剂量的增加呈正相关,但各剂量组差异无统计学意义。结论:梅术溃结灌肠汤可显著降低IL-6、MDA,升高IL-10水平,对溃疡性结肠炎有良好的治疗作用。

Objective： To explore the part of mechanism of action of Mei colitis and enema soup on treatment of ulcerative colitis. Methods： Experimental rats were randomly divided into the blank group, the model group, low, middle, high dose drug group, 10 respectively; Using radiation immunifaction method to measure serum IL-6, IL-10, using colorimetric method to measure MDA. Results： The IL-6,IL-10 and MDA content of the blank group and model group has statistically significant difference（P〈0.01）, while the drug groups and model group also has statistically significant difference （P〈0.01）. and the blank group was not statistically significant difference; The IL-6 of high dose group was higher than low dose group, the MDA of low dose group was more than high dose group, the difference was significant （P〈0.05）, and the latter was negatively correlated with increase of dose; IL-10 was positively associated with increase of dose, but there was not significant difference of the different dose group. Conclusion： Mei colitis and enema soup can significantly reduce IL-6, MDA, rise IL-10 level, has good curative effect on ulcerative colitis.