清心丸对小鼠抗焦虑行为影响的实验研究

Experimental study of the effects of Qingxin pilula on the behavior in EPM mice

目的通过高架十字迷宫小鼠的行为学试验,观察清心丸对小鼠的抗焦虑作用。方法选用小鼠50只,分为5组,每组10只。分为空白对照组,地西泮阳性对照药组,清心丸高、中、低剂量组,各组分别给予与清心丸等体积蒸馏水、地西泮片(2 mg/kg)、清心丸(4.0、2.0、1.0 g/kg)。灌胃给药,每日1次,连续7 d。7 d后观察清心丸高、中、低剂量对小鼠进入开放臂和封闭臂的总次数(OE+CE)、进入开放臂次数百分比(OE%)、开放臂停留时间比例(OT%)的影响。结果清心丸中、高剂量组的进入开放臂次数(OE)均明显增加(P<0.05),清心丸中、高剂量组与地西泮组在开臂停留时间(OT)分别占进入两臂总次数和在两臂停留总时间OE%和OT%均比对照组明显增加(P<0.05),而各组OE+CE的总次数未见明显变化。结论清心丸具有较强的抗焦虑作用。

Objective To observe Qingxin pilula anti - anxiety effects through the behavior experiment ot EPM mice. Methods Using the mice elevated cross labyrinth, selecting diazepam as the positive control medicine,to observe the effects of Qingxin Pilula of high, medium and low doses of mice into the open arms of the total number and enclosed arm （ OE ＋ CE）, entering open arms percentage （OE%）, open arms time scale （OT）. Results The number of middle and high dose group of Qingxin Pilula into the open arms （OE） were significantly increased（ P 〈 0. 05 ）. The Qingxin Pilula middle, high - dosage groups and diazepam group in the open arms retention time percentage （OE % and OT % ）respectively were significantly increased than control group（ P 〈 0.05 ）, and each of the to- tal number of OE ＋ CE without obvious changes. Conclusion The Qingxin pilula has strong anti - anxiety effects.