不同途径给予重组人肠三叶因子对烧伤后小鼠肠黏膜屏障功能的影响

Effects of different administration pathways of recombinant human intestinal trefoil factor on intestinal mucosal barrier function after burn injury

目的探讨重组人小肠三叶因子(rhITF)对烧伤后肠黏膜屏障功能的影响。方法建立30%体表面积Ⅲ度烧伤小鼠模型,将104只BALB/c小鼠随机分为正常对照组(C组,n=8)、烧伤对照组(B组,n=32)、肠三叶因子灌胃给药组(IG组,n=32)和rhITF皮下注射组(SC组,n=32)。观察伤前及烧伤后1、3、5、7d小鼠肠黏膜屏障功能的变化及不同途径给予rhITF对其的影响。结果烧伤后肠黏膜损伤指数、通透性、血浆二胺氧化酶(DAO)活性明显高于C组,而肠上皮细胞增殖指数和肠黏膜厚度则显著降低。与B组比较,IG组和SC组小鼠的损伤指数、通透性和DAO活性明显降低(P<0.05),黏膜厚度显著增加(P<0.05)。与SC组比较,IG组的疗效更明显(P<0.05)。结论 rhITF能有效减轻烧伤后肠道损伤,维护肠黏膜屏障功能,通过胃肠途径给予肠三叶因子的疗效更佳。

Objective To observe the effects of recombinant human intestinal trefoil factor（rhITF） on intestinal mucosal barrier function after severe burn injury. Methods The mouse model of 30% body surface area third degree burns was established. 104 BALB/c mice were randomly divided into four groups： namely normal control（C, n = 8）, burned control （B, n = 32）, rhITF intragastric administration（IG,n = 32） and rhlTF subcutaneous injection （SC, n = 32） groups. The changes of intestinal mucosal barrier function and the efficacy of different rhITF administration pathways were analyzed before burn and on post-burn 1,3,5,7 d. Results After burn injury, the level of mucosa damage index, the intestinal mucosal permeability and the activity of diamine oxidase （DAO） in three burned groups were significantly higher than those in the group C,while the enterocyte proliferation index and the intestinal mucosa thickness were remarkably decreased. Compared with the group B,the damage index, the intestinal mucosal permeability and the activity of DAO in the IG and SC groups were obviously reduced（P〈0.05） and the mucosa intestinal mucosa thickness was significantly increased（P〈0.05）. Compared with the group SC,the efficacy in the group IG was more apparent（P〈 0.05）. Conclusion rhITF administration can effectively minimize the intestinal damage after burn injury,protect the intestinal mucosal barrier, in. Comparison,rhITF intragastric administration has better efficacy.