补肾益精法中药复方对硬皮病小鼠皮肤纤维化的影响

Effect of Bushen Yijing Formula on Dermal Fibrosis in Scleroderma Model Mice

目的:研究补肾益精法中药复方对硬皮病小鼠皮肤纤维化表型的影响。方法:使用博来霉素诱导建立硬皮病小鼠模型,分别采用补肾益精法中药复方(中药复方组)、生理盐水(模型组)处理硬皮病模型小鼠,共3周,并设正常对照组。HE染色观察皮肤病理改变;使用实时定量逆转录-聚合酶链反应(RT-PCR)技术,测定各组小鼠I型胶原α2(COLⅠα2)基因表达情况。结果:成功建立硬皮病小鼠模型,皮肤病理HE染色显示,中药复方组小鼠皮肤纤维化有不同程度改善。模型组小鼠COLⅠα2基因表达较正常对照组高(P<0.01);经补肾益精法处理后,中药复方组小鼠COLⅠα2基因表达减少,与正常对照组比较,差异无显著性意义(P>0.05)。结论:补肾益精法中药复方可改善硬皮病模型小鼠皮肤纤维化程度。

Objective： To study the effect of Bushen Yijing formula, which has the action of tonifying kidney and nourishing essence, on dermal fibrosis in sc）eroderma model mice. Methods： Rat models of scleroderma were induced by bleomycin, and then were treated with Bushen Yijing formula or normal saline for three weeks. At the same time, normal control group was set. The pathological change of mice skin was observed by HE staining method, and the expression of collagen 1α 2（COL 1α 2） was detected by real-time quantitative reverse transcription polymerase chain reaction（RT-PCR）. Results. Animal models of scleroderma were successfully established. The results of HE staining showed that dermal fibrosis of Bushen Yijing formula group was improved. COL 1α2 expression level in the model group was higher than that in the normal control group（P 〈 0.01）, and was decreased in Bushen Yijing formula group after treatment （P 〉 0.05 compared with that in the normal control group）. Conclusion： Bushen Yijing formula can relief dermal fibrosis in scleroderma model mice.