升清降浊胶囊对高糖诱导腹膜纤维化大鼠HMGB_1和TGF-β_1的影响

Effect of Shengqing Jiangzhuo Capsule on High-mobility Group Box 1(HMGB_1)and Transforming Growth Factor-β_1 Contents in Peritoneal Fibrosis Rats Induced by High Glucose

目的:观察升清降浊胶囊对高糖诱导的腹膜纤维化大鼠分泌高迁移率族蛋白1(HMGB)1和转化生长因子β1(TGF-β)1的影响。方法:将SD雄性大鼠40只随机分成4组:空白组、模型组、低剂量组、高剂量组各10只。空白组与模型组予5mL生理盐水灌胃,低剂量组予升清降浊胶囊50mg/(kg.d)、高剂量组予升清降浊胶囊100mg/(kg.d),由生理盐水稀释至5mL后灌胃,每天1次,共4周。分别于实验第3、7、14、28天时大鼠尾静脉取血检测HMGB1和TGF-β1,第28天处死各组大鼠,留取壁层腹膜组织做HE及Masson染色,测量腹膜厚度。结果:模型组HMGB1及TGF-β1的含量、腹膜致密层厚度均高于空白组(P<0.05);中药低剂量组HMGB1及TGF-β1含量、腹膜致密层厚度均低于模型组(P<0.05);中药高剂量组HMGB1及TGF-β1含量、腹膜致密层厚度降低更明显,与低剂量组比较,差异均有显著性意义(P<0.05)。结论:升清降浊胶囊可以降低HMGB1、TGF-β1含量,降低腹膜致密层厚度,且随着剂量增加效果更明显,提示升清降浊胶囊对延缓腹膜纤维化有一定意义。

Objective： To investigate the effect of Shengqing Jiangzhuo capsule （SJC） on high mobility group box1 （HMGB1） and transforming growth factor-β1（TGF-β1）contents in peritoneal fibrosis rats induced by high glucose. Methods： Forty SD male rats were randomly divided into four groups： blank control group, model group, low-dose SJC （50 mg·kg-1·d-1） group and high-dose SJC （100 mg·kg-1 ·d-1） group, 10 rats in each group. Rats in the blank control group and model group were treated with 5mL of normal saline. The medication lasted four weeks. The contents of HMGB1 and TGF-β 1 in the blood was detected at the 3rd, 7th, 14th and 28th day of experiment, the changes of peritoneal thickness were measured through hematoxylin-eosin （HE） staining and Masson trichrome staining. Results. The contents of HMGB1 and TGF- 131 in the model group were significantly higher than that in the blank control group （P 〈 0.05）. The contents of HMGB1 and TGF- β 1 in the low-dose SJC group were significantly lower than those in the model group （P 〈 0.05）, and HMGB1 and TGF- β1 contents in high-dose SJC group were much lower than that in low-dose SJC group（P 〈 0.05）. Conclusion. Shengqing Jiangzhuo capsule can reduce the contents of HMGB1 and TGF- β1 in peritoneal fibrosis rats induced by high glucose by dose-dependent manner, indicating that the capsule can be expected to delay the progression of peritoneal fibrosis.