滋阴清热中药预防小鼠单倍体移植后慢性移植物抗宿主病实验研究

Experimental Study of Preventive Effect of Yin-nourishing and Heat-clearing Chinese Medicine on Chronic Graft-versus-host Disease in Mice with Haploidentical Bone Marrow Transplantation

目的:观察预防性应用滋阴清热复方中药对慢性移植物抗宿主病(cGVHD)模型小鼠的影响。方法:小剂量中药组、中剂量中药组、大剂量中药组受鼠从诱导起分别给予相应剂量的滋阴清热中药,阴性及阳性对照组给予等剂量生理盐水,诱导后第2、5、8、12周,检查骨髓细胞中的Y染色体数量,进行嵌合体分析。诱导后每3天观察受鼠的体重、毛发变化并评分。诱导后100天断颈处死小鼠,观察皮肤、肝脏、回盲端小肠的病理变化并评分。结果:阳性对照组和不同剂量中药干预组均可形成较为稳定的供受者混合嵌合体,给予滋阴清热中药预防后,大剂量中药组、中剂量中药组cGVHD的发病率降低,小鼠毛发改变、体重变化和肝脏、皮肤、肠道病理改变评分与阳性对照组比较,差异均有统计学意义,小剂量中药组上述指标与阳性对照组比较,差异均无统计学意义。结论:适当剂量的滋阴清热中药可预防单倍体相合移植后cGVHD的发生。

Objective. To observe the effect of Chinese medicine of nourishing yin and clear heat for prevention of chronic graft-versus-host disease （cGVHD） in mice. Methods： Mice in the Chinese medicine groups were administrated with high-dose, middle-dose and low-dose yin-nourishing and heat-clearing Chinese medicine respectively from the day of model induction. Positive control group and negative control group were administrated with the same volume Of normal saline. At the 2rid, 5th, 8th, 12th week of model induction, the number of Y chromosome in bone marrow cells was detected for the analysis of to chimerism. The body weight, hair and incidence of cGVHD were observed every 3 days. One hundred days after model induction, the pathological changes of mice skin, liver and intestine were observed. Results. Stable chimerism formed in all Chinese medicine groups and positive control group. Incidence of cGVHD in high-dose and middle-dose Chinese medicine groups was lower than that in low-dose Chinese medicine group and positive control group. As for the symptomatic grading and pathological grading, statistical difference was found between high-dose Chinese medicine group and positive control group while not found between low-dose Chinese medicine group and positive control group. Conclusion： Appropriate dose of Chinese medicine of nourishing yin and clear heat can prevent sGVHD in mice with haploidentical bone marrow transplantation.