非霍奇金淋巴瘤患者外周血CD4^＋CD25^highCD127^low调节性T细胞水平与其临床特点的关系

Correlation between peripheral blood CD4＋CD25highCD127low regulatory T cell and clinical characteristics of patients with non-Hodgkin＇s lymphom

目的探讨外周血CD4＋CD25highCD127low调节性T细胞（Treg细胞）水平与非霍奇金淋巴瘤（NHL）的关系。方法采用流式细胞术检测123例初治NHL患者、41名健康成年人外周血c功c础}CD笛，IIreg水平，并分析其与临床因素的关系。结果健康成年人外周血CD4＋CD25highCD127lowTreg水平中位数为5,0％（四分位数间距为2．7％），初诊NHL患者中位数为7．2％（四分位数间距为3．8％），两者之间差异有统计学意义（Z=～2．816，P=0．001）。初诊NHL患者外周血Treg水平在男性患者中中位数为7．2％（四分位数间距为7．4％），在女性患者中中位数为6．1％（四分位数间距为3．8％），差异有统计学意义（Z=-2．729，P=0．006）；乳酸脱氢酶（LDH）增高组中位数为7．8％（四分位数间距为5．9％），LDH正常组中位数为7．0％（四分位数间距为3．3％），差异具有统计学意义（Z=-2．623，P：0．008）；III～IV期患者中位数为7-3％（四分位数间距为5．3％），I一Ⅱ期患者中位数为5．9％（四分位数间距为3．7％），差异有统计学意义（Z=-2．057，P=0．04）；而NHL患者在不同年龄、IPI评分、结外病变、体能状况、大包块、病理类型、B症状等之间外周血Treg水平差异无统计学意义（均P〉0．05）。结论NHL患者体内存在免疫抑制，在男性患者、LDH增高患者及分期为Ⅲ～Ⅳ期患者中CD4＋CD25highCD127lowTreg水平明显增高。

Objective To explore the relationship between CD4＋CD25highCD127low Treg level of peripheral blood and NHL.Methods The level of peripheral blood CD4＋CD25highCD127low Treg of 123 newly diagnosed NHL D low and 41 healthy adults were test by flow cytometry.Results The average peripheral blood CD4＋CD25highCD127low Treg level were 5.0 % （2.7 %） and 7.2 %（3.8 %） in healthy adults and newly diagnosed NHL patients, respectively. The difference between the two groups was statistically signifieant（Z = -2.816 P=0.001）. The peripheral blood Treg level was significantly higher in the male NHL patients than that in the female （7.4% vs 6.1% ,Z= -2.729,P=0.006）.The peripheral blood Treg level was significantly higher in elevated LDH patients than that in normal LDH patients（7.8% vs 7.0%,Z = - 2.623 P=0.008）. The peripheral blood Treg level was significantly higher in stage III-IV patients than that in stage I -II patients （7.3% vs 5.9%,Z= -2.057 P=0.04）. There was no significant relationship between peripheral blood Treg level and age, IPI score, extranodal involvement,performance status,bulky disease,pathologic subtype or B symptom （P 〉0.05）. Conclusions NHL patients are in an immunosuppresive statue. Patients of male, with inreased LDH level or in stage III -IV have higher peripheral blood CD4＋CD25highCD127low Treg level.