三种浆果提取物体外抗氧化活性研究

Study on in vitro antioxidant activities of extraction from three berries

目的:探讨笃斯越橘、紫叶稠李和山桃稠李三种浆果提取物的体外抗氧化作用,并比较三者的体外抗氧化活性。方法:将三种浆果的烘干粉末分别用蒸馏水和60%(体积分数)乙醇浸提,然后将它们稀释为五个梯度后,采用分光光度法、水杨酸比色法、邻苯三酚自氧化法、硫代硫酸钠滴定法、铁氰化钾还原法测定三种浆果提取物对DPPH自由基、羟基自由基、超氧阴离子自由基、过氧化氢的清除能力以及总还原能力。结果:这三种浆果提取物都具有较强的体外抗氧化作用。其中笃斯越橘醇提物最强DPPH.清除率为93.50%、紫叶稠李水提物最强.OH清除率为95.45%、紫叶稠李水提物最强O2-.清除率为88.81%、山桃稠李水提物最强H2O2清除率为70.71%,山桃稠李水提物总还原能力最强为1.901。结论:三种浆果提取物对4种自由基清除率和总还原能力都具有明显的剂量依赖关系,并且同种浆果的不同提取物对自由基的清除率也不同。

Objective=To research the berry crude extracts in vitro antioxidative activity from Vaccinium uliginosum, Padus virginiana,Prunus maackii and compare the in vitro antioxidant activity of three berries. Methods：The three substances were dried powder with aqueous extraction and 60% ethanol extraction,and they were then diluted to five different concentrations. Each concentration was measured by spectrophotometry,salicylic acid colorimetric method,self-oxidation of pyrogallol,sodium thiosulfate titration and potassium ferricyanide reduction method to determine the ability to scavenge DPPH radicals,hydroxyl free radical,super-oxideanion radicals, hydrogen peroxide and total reduction ability respectively. Result.These three substances had a strong in vitro antioxidant effect. Among these three substances,the highest DPPH. clearance rate of the ethanol extract of Vacciniun uliginosum was 93.5% ,the highest .OH clearance rate of the aqueous extract of Padus virginiana was 95.45% ,the highest O2-. clearance rate of the aqueous extract of Padus virginiana was 88.81% ,the highest H2O2 clearance rate of the aqueous extract of Prunus maackii was 70.71% ,the highest total reduction ability of the aqueous extract of Prunus maackii was 1.901. Conclusion=Three kinds of berries powder extraction which scavenged four kinds of radical scavenging and total reducing power were increased with increasing concentration of substance,and had significant dose-dependent. The same material under the same conditions,the change of the leach liquor will affect clearance rate of free radical change.