鲜马铃薯直接生产茁霉多糖中试工艺研究

Pilot scale production for pullulan polysaccharide with fresh potatoes

在茁霉多糖小试生产工艺的基础上设计了5L中试生产工艺方案,分别研究了接种量和转速对中试发酵的影响,对比研究了中试和小试发酵液在生物量、多糖产量、蛋白含量及色素等各项指标上的差异,结果表明:接种量对中试发酵有一定影响,其中以12%的接种量效果较好;与小试发酵不同,中试发酵在搅拌速度上应采用变速搅拌方式,即前期发酵采用160r.min-1较低搅拌速度,在后期发酵时应采用200r.min-1的搅拌速度;两种发酵方式比较,在相同的发酵时间内,生物量和多糖产量两项指标,中试发酵均高于小试发酵,而蛋白质和色素产量较小试发酵低,确定的中试工艺条件为:装液量3.5L,接种量12%,初始pH6.5,温度30℃,发酵时间5d,在此工艺下制得的发酵液中多糖含量达到3.66g/L。

Based on the production technology of laboratory fermentation experiment of pullulan polysaccharide, 5L pilot fermentation was designed. The effects of inoculation and stirring rate on the pilot fermentation were studied,the differences of the biomass,polysaccharide,protein content and pigment and other indicators were also studied between the pilot and laboratory fermentation. The results showed that：the inoculation had a certain influence on the fermentation,of which 12% was better and different from laboratory fermentation, pilot fermentation should be used to stir by mixing method（160r.min 1 was used early,and 200r. min-1 was used latter）. Compared with the two methods,biomass and polysaccharide of pilot fermentation were higher than the laboratory in the same period of fermentation,and protein and pigment were lower. The optimum conditions were determined as followsliquid volume 3.5L,inoculation 12% ,initial pH6.5,temperature 30℃,fermentation time 5d. The content of polvsaccharide in fermented liquid were 3.66q/L.