摘要
Intrauterine growth retardation （IU- GR） causes significantly negative effects on the meth- ylation status of genes related to cell apoptosis com- pared with normal body weight （NBW） piglets. Thus, the objective of the present study was to exam- ine the effects of maternal dietary folic acid supple- mentation on genes expression profile for hepatic ap- optosis in IUGR and NBW piglets. Twenty four York- shire gilts were allocated randomly to one of the two diets ： control （ C, folic acid 1.3 mg/kg） or folic acid supplementation （ FS, folic acid 30 mg/kg） after mat- ing. Gene expressions in liver samples were deter- mined and revealed that the mRNA expressions of p53 ,BCL-2 associated X protein （Bax）, and Cyclin- dependent kinase inhibitor 1A （CDKN1A） were up-regulated in IUGR piglets compared with NBW pig- lets fed C diets,but could be reversed by maternal fo- lic acid supplementation. The expressions of vascular endothelial growth factor （VEGF）, Serine-protein Ki- nase-Ataxia Telangiectasia Mutated （ATM） ,and Cad- herin-associated protein-beta-catenin 1 （ CTNNB1 ） were influenced by maternal folic acid supplementa- tion significantly, but were not influenced by birth weight. Expression of p53 binding protein-MDM-2 （ MDM-2 ） remained unchanged. In conclusion, these results demonstrated that maternal folic acid supple- mentation could exert positive effects on genes related to apoptosis in IUGR and NBW piglets, which might facilitate their postnatal health and growth perform- alice.
Intrauterine growth retardation （IU- GR） causes significantly negative effects on the meth- ylation status of genes related to cell apoptosis com- pared with normal body weight （NBW） piglets. Thus, the objective of the present study was to exam- ine the effects of maternal dietary folic acid supple- mentation on genes expression profile for hepatic ap- optosis in IUGR and NBW piglets. Twenty four York- shire gilts were allocated randomly to one of the two diets ： control （ C, folic acid 1.3 mg/kg） or folic acid supplementation （ FS, folic acid 30 mg/kg） after mat- ing. Gene expressions in liver samples were deter- mined and revealed that the mRNA expressions of p53 ,BCL-2 associated X protein （Bax）, and Cyclin- dependent kinase inhibitor 1A （CDKN1A） were up-regulated in IUGR piglets compared with NBW pig- lets fed C diets,but could be reversed by maternal fo- lic acid supplementation. The expressions of vascular endothelial growth factor （VEGF）, Serine-protein Ki- nase-Ataxia Telangiectasia Mutated （ATM） ,and Cad- herin-associated protein-beta-catenin 1 （ CTNNB1 ） were influenced by maternal folic acid supplementa- tion significantly, but were not influenced by birth weight. Expression of p53 binding protein-MDM-2 （ MDM-2 ） remained unchanged. In conclusion, these results demonstrated that maternal folic acid supple- mentation could exert positive effects on genes related to apoptosis in IUGR and NBW piglets, which might facilitate their postnatal health and growth perform- alice.
基金
supported by Program of Changjiang Scholars and Innovative Research Team in University of China(IRT0555-5)
