大孔树脂分离纯化巴豆中木兰花碱工艺研究

Separation and Purification of Magnoflorine from Croton Tiglium by Macroporous Resins

目的:优化大孔树脂分离纯化巴豆中木兰花碱的工艺条件。方法:采用HPLC法测定木兰花碱,筛选AB-8,HPD400,ADS-17,NKA-9,D101树脂对木兰花碱的吸附和解吸附能力,并优化分离纯化条件。结果:优选出D101树脂,上样液浓度为生药1.5g/mL,吸附流速1.5BV/h,药材量:树脂量为6∶1;分离纯化条件为:先用4BV水冲洗除去不吸附杂质,再用3BV 8%乙醇除去大极性干扰杂质,最后用4BV 23%乙醇冲洗收集目标滤液。结论:该方法简便易行,适用于巴豆中木兰花碱的精制与纯化。

Objective： To study the optimal optimally purified process of magnoflorine extracted from Croton tiglium with macroporous res- ins. Methods： The HPLC was used to analyze the content of magnoflorine,the adsorption and desorption performances of AB-8, HPD400, ADS-17,NKA- 9,D101 macroporous resins were compared and then optimize purification conditions. Results： D101 resin was selected because it gave the most effective of separation. The optimum process for the purification was as follows： the concentration of sample solution was 1. 5 g/mL; adsorption flow rate was 1.5 BV/h; crude Medicinal materials weight than reisn weight for six. 4 BV water 3 BV 8 % ethanol and 4 BV 23 % ethanol were used as etuant of Croton tiglium respectively. Portions of 23 % ethanol were collected. Conclusion： This method is simple and feasible with good effect of enrichment in magnoflorine from Croton tiglium.