附子及其与三种常见药配伍对关木通所致慢性肾病小鼠的影响

The Effects of Fuzi Combination With Three Common Drugs on Chronic Renal Failure Mice Induced by Aristolochic Acid

目的:研究附子、依那普利、别嘌醇和氨基胍对关木通所致慢性肾病模型的影响,探究其肾脏保护作用及与相关氧化还原物质的关系。方法:将雄性小鼠用关木通造模两周后随机分组:正常对照组,关木通模型组,关木通加各治疗药物组。实验结束时,测定血清肌酐、尿素氮、肾组织蛋白、丙二醛、尿酸、谷胱甘肽、谷氨酰基转移酶等的含量,并记录肾、睾丸系数。结果:附子单用能够显著降低肾系数、血肌酐、尿素氮水平,降低肾尿酸,且显著升高肾脏谷氨酰基转移酶含量;依那普利可以显著降低血肌酐、尿素氮、肾组织谷胱甘肽含量,升高肾谷氨酰基转移酶活性,进一步升高肾尿酸。别嘌醇能够显著降低肾系数、血尿素氮,显著升高肾蛋白含量并且进一步升高肾谷胱甘肽含量。氨基胍能够明显降低肾系数、并且进一步升高血肌酐、尿素氮。结论:该模型中肾脏氧化还原处于较低状态,机体的氧化还原平衡出现紊乱,氧自由基减少,还原性物质增多,尿酸代谢异常。实验结果表明附子在该模型上表现出一定的治疗作用,与抑制尿酸代谢的药物合用能够起到较好肾脏保护作用。但是在发病过程中抑制一氧化氮的生成能够加重病情。依那普利改善肾脏血流量同样能够起到缓解病情作用。

Objective： To study the aconite, enalapril, allopurinol and aminoguanidine compatibility on aristolochic acid induced chronic renal failure model, and to find out the renal protective effects with relevant redox material relationship. Methods. Two ex- periments were conduct. Male mice were randomLy divided into control group, model group and treatment group after intragastric administration aristolochic acid for two weeks. At the end of the experiment, to detected the serum creatinine（Scr）, blood urea ni- trogen（BUN）, concentration of protein,urlc acid（UA）, malondialdehyde （MDA）, glutathione （GSH）, γ-glutamyl transpeptidase （γ-GT） in kidney, and record the kidney index, testis index. Results： Aconite can significantly reduce renal index, Scr, BUN, and content of UA in kidney and significantly increased content of γ-GT in kidney; enalapril can significantly reduce the BUN,Scr, content of GSH in kidney, elevated γ-GT activity in kidney, and further increased UA in kidney. Allopurinol can significantly reduce kidney index, serum BUN, increased protein in kidney, while further increase the GSH content in kidney. Aminoguanidine can significantly reduce kidney index, while further increase the Scr, BUN levels. Conclusion ： Renal redox reaction of mice in model group were inhibited, and the balance of redox was disordered which oxygen free radicals reduced, while the reductive substances increase, uric acid metabolism. Experimental result shows that aconite can exert a certain therapeutic effect. Inhibiting the uric acid metabo- lism can play a positive role in protecting kidney. Inhibition of generation of NO can aggravate injury. Enalapril improves rate of blood flow of renal can also play a positive role in the remission of the injury.