腾冲热海嗜热芽孢杆菌的分离鉴定

Isolation and Characterization of Thermophilic Bacillus from in Tengchong Rehai

从云南腾冲热海温泉中分离得到26株嗜热芽孢杆菌菌株,对其中一株嗜热芽孢杆菌菌株NHH4进行电镜形态、生长特征、碳源、氮源等分析。该菌株细胞形态为杆状,产芽胞,革兰氏染色阳性,严格好氧,其最适生长温度为55℃,最适为pH7.5。能利用葡萄糖、蔗糖、D-果糖、D-甘露糖,不能利用麦芽糖、乳糖、D-木糖、L-鼠季糖。通过对其16S rRNA基因序列的系统发育分析表明,NHH4与Bacillus licheniformis strain N8的序列相似性为99%,将此菌株鉴定为嗜热地衣芽胞杆菌菌株。

26 Thermophilic Bacillus was obtained according to the disassociation from Tengchong hot springs.And one of the strains,NHH4 is selected to analyze its morphologic characteristics,development features,nitric and carbon sources.The cytomorphology of the bacillus is nemaline with gemma,gram-positive,oxygen-loving.And the most suitable temperature for its development is 55℃,the PH value is 7.5.It can use glucose,sucrose,D-frucose,D-mannopyranose,but can＇t use maltose,lactose,D-xylose,L-rhamnose.The analysis on the phylogeny of 16srRNA＇s gene sequence indicates that the similarity of NHH4 and Bacillus licheniformis strain N8 is 99%,so the bacillus is identified as thermophilic lichen-bacillus.