摘要
目的探讨d,JL肺炎支原体肺炎(mycoplasma pneumoniae pneumonia,MPP)不同病期T细胞亚群、免疫球蛋白、补体的变化及其临床意义。方法应用流式细胞术、免疫散射比浊法检测28例MPP患儿急性期及恢复期外周血T细胞亚群(CD3、CD4、CD8)、免疫球蛋白(IgG、IgA、IgM)、补体(c3、C4)水平,并与25例健康儿童(对照组)进行比较。结果MPP患儿急性期外周血CD3、CD4、CD8、CD4/CD8分别为(58.71±11.63)%、(32.36±8.06)%、(28.19±6.23)%、1.15±0.41,恢复期分别为(61.29±10.17)%、(34.14±7.22)%、(26.47±6.01)%、1.29±0.37。急性期与恢复期MPP患儿CD4、CD4/CD8比值均低于对照组[(39.53±6.16)%、1.83±0.49],CD8水平高于对照组(1.83±0.49),差异均有统计学意义(P均〈0.01)。急性期CD3水平与对照组[(63.03±12.32)%]比较差异有统计学意义(P〈0.01),而恢复期无明显差异(P〉0.05)。MPP患儿急性期外周血免疫球蛋白与对照组比较,血清IgG[(14.50±3.86)∥L]、IgM[(1.67±0.56)g/L]与对照组[(7.92±2.62)g/L、(1.06±0.32)g/L]比较明显增高,c3[(0.83±0.42)g/L]水平低于对照组[(1.37±0.33)∥L],差异均有统计学意义(P〈0.05);而IgA、C4水平与对照组比较差异无统计学意义(P〉0.05)。结论MPP患儿存在细胞免疫和体液免疫失调。检测T细胞亚群、免疫球蛋白、补体的变化,有利于判断临床治疗效果,为临床应用免疫调节剂提供理论依据。
Objective To investigate the changes and functions of T lymphocyte subsets, immune globulin and complement in children with mycoplasma pneumoniae pneumonia (MPP) on different disease stages. Methods The levels of T lymphocyte subsets of CD3, CD4, CD8 and immunoglobulin ( IgG, IgA IgM) ,and complement (C3, C4 ) in the peripheral blood were detected on acute and recovery stages in 28 children with MPP by flow cytometry and immune nephelometry. Twenty-five healthy children were recruited as control group. Results Among these subjects of MPP children on acute stage, the levels of CD3, CD4, CD8,and CD4/CD8 in the peripheral blood were (58.71 _± 11.63)%, (32.36 ± 8.06)%, (28.19 ± 6. 23 ) % and 1.15 ± 0. 41 respectively, and on recovery stage, the levels of CD3, CD4, CD8, and CD4/CD8 were (61.29 ± 10. 17) %, (34. 14± 7. 22) %, (26.47 ± 6. O1 ) %, and 1.29 ± 0. 37 respectively. Both on a- cute stage and on recovery stage of MPP children, the levels of CD4, CD4/CD8 were significantly lower than those in control group E (39. 53 ± 6. 16) %, 1.83 ± 0.491, and CD8 was significantly higher compared to the control group ( 1.83 ± 0.49 ), P 〈 0. 01. CD3 were lower than that in control group E ( 63.03 ± 12. 32 ) %] on acute stage ( P 〈 0. 01 ), and no significant difference on recovery stage ( P 〉 0. 05 ). During the acute stage of MPP,IgG E (14. 50 ±3.86) g/L3 and IgM g/L (1.67 ±0. 56) g/L3 were obviously higher than those in con- trol group (7.92±2.62) g/L,(1.06±0.32) g/L,P〈0.01),and C3 I(0.83 ±0.42) g/L3 were obvi- ously lower compared to the control group [ ( 1.37 ±0. 33) g/L,P 〈0. 053. There were no significant differ- ences of IgA and C4 between MPP and control groups ( P 〉 0. 05 ). Conclusion Children with MPP had cel- lular immune and humoral immune disorders. Through the detection of T lymphocyte subsets, immunoglobu- lin and complement,it will be helpful to judge the effectiveness of clinical treatment, which provides a theo- retical basis for the clinical application of immune regulators.
出处
《中国小儿急救医学》
CAS
2012年第3期245-247,共3页
Chinese Pediatric Emergency Medicine
关键词
肺炎支原体肺炎
T细胞亚群
免疫球蛋白
补体
儿童
Mycoplasma pneumoniae pneumonia
T lymphocyte subsets
Immunoglobulin
Comple-ment
Children