摘要
目的和方法 为研究细胞因子在实验性变态反应性脑脊髓炎 (EAE)及多发性硬化 (MS)发病机制中的作用 ,用 CTL L细胞株滴定法检测了 EAE动物脾细胞培养上清中白细胞介素 - 2 (IL- 2 )的生物学活性 ,用EL ISA分别检测了白细胞介素 - 4 (IL - 4 )及干扰素 -γ(IFN-γ)水平。结果 EAE组 IL - 2活性及 IFN-γ水平均显著高于正常对照组 (均为 P<0 .0 5) ,而其 IL- 4水平显著低于对照组 (P<0 .0 1)。结论 IL- 2、IFN- γ等细胞因子参与了 EAE的发病 ,并对 IL- 4的产生有抑制作用。
Objective and Methods To investigate cytokine effects on EAE pathogenesis, IL 2 bioactivity in the supernatant of cultured spleen cells of EAE model was assayed with CTLL cell line titration; IL 4 and IFN γ levels were detected with ELISA. Results IL 2 bioactivity and IFN γ levels in EAE group were significantly higher than that of control group( P <0 01; P <0 05). IL 4 level was significantly lower than that of control group( P <0 01). Conclusion Some kinds of cytokines(IL 2 and IFN γ) were showed their contribution to the pathogenesis of EAE, and inhibiting IL 4 production.
出处
《中国神经免疫学和神经病学杂志》
CAS
2000年第1期45-48,共4页
Chinese Journal of Neuroimmunology and Neurology
基金
:黑龙江省自然科学基金! (0 - 96 2 2 )