复合涤纶毡型韧带替代家犬膝关节前交叉韧带的实验研究

The experimental study of the dilurn polyester felt type artificial ligament substitute dogs'knee join anterior cruciate ligament

目的观察复合涤纶毡型韧带替代家犬膝关节前交叉韧带(Anterior Cruciate Ligament ACL)的组织相容性和生物力学变化。方法用复合涤纶毡型人工韧带植入家犬膝关节替代前交叉韧带,观察犬膝关节活动情况,分别于术后6、9、12周取组织标本进行肉眼、光镜、电镜观察及力学强度测试。结果术后6周涤纶毡型韧带被纤维组织呈"岛屿"状分隔,散在成纤维细胞生长活跃,韧带与周围组织分界不清。术后9周"岛屿"状分隔的纤维组织已相连接成片状,完全充填韧带的微孔,韧带周边胶原化,已无炎性细胞浸润。术后12周HE染色显示韧带微孔内及周边的胶原化区域较9周时更加均匀致密,韧带周围的胶原带与伸入韧带中间的胶原化纤维组织连接成片状,盲区逐渐缩小直至消失。韧带抗拉强度不随时间的延长而发生衰减。结论复合涤纶毡型韧带的网状孔隙结构有利于组织长入,具有良好的生物相容性和力学性能,能用于重建前交叉韧带。

Objective To study the compatibility in animal and biomechanics characteristic in knee join of the dilurn polyester felt type artificial ligament.Methods The anterior cruciate ligament（ACL） of dogs＇knee was substituted by dilurn polyester felt type artificial ligament.Specimens were collected 6,9 and 12 weeks after operation to measure their mechanical characteristics and be observed by naked eye,optical microscope and electron microscope.Results After implantation,the dilurn polyester felt type artificial ligament（ACL） was islanded by fibrous tissue after 6 weeks,the scattered fibroblast was growing activity,the ligament had no clear boundary with the surrounding tissue.After 9 weeks,the fiber organization had connected into flake,complete filling of the ligament micro hole and around the ligament surrounding had no inflammatory cells and will be collagen.After 12 weeks,HE dyed shows it becomes more uniform than 9 weeks in side of and surroounding the microporous ligament.Collagen and collagen fiber with the organization was connected into flake.Blind area gradually narrowed until vanishing.The tensile strength of ligament will not weakening with the extension of time.Conclusion There is abundant holes and network structure in artificial ligament which benefits to growth and recovery of tissue.It can be used in repairing and reconstruction of ligament tissue of human body.